Rapid, cost-effective DNA quantification via a visually-detectable aggregation of superparamagnetic silica-magnetite nanoparticles
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- Liu, Q., Li, J., Liu, H. et al. Nano Res. (2014) 7: 755. doi:10.1007/s12274-014-0436-9
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DNA and silica-coated magnetic particles entangle and form visible aggregates under chaotropic conditions with a rotating magnetic field, in a manner that enables quantification of DNA by image analysis. As a means of exploring the mechanism of this DNA quantitation assay, nanoscale SiO2-coated Fe3O4 (Fe3O4@SiO2) particles are synthesized via a solvothermal method. Characterization of the particles defines them to be ∼200 nm in diameter with a large surface area (141.89 m2/g), possessing superparamagnetic properties and exhibiting high saturation magnetization (38 emu/g). The synthesized Fe3O4@SiO2 nanoparticles are exploited in the DNA quantification assay and, as predicted, the nanoparticles provide better sensitivity than commercial microscale Dynabeads® for quantifying DNA, with a detection limit of 4 kilobase-pair fragments of human DNA. Their utility is proven using nanoparticle DNA quantification to guide efficient polymerase chain reaction (PCR) amplification of short tandem repeat loci for human identification.
Keywordssilica/magnetite core-shell superparamagnetic DNA quantification polymerase chain reaction (PCR)
© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2014