Nano Research

, Volume 3, Issue 8, pp 557–563

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

Authors

  • Miao Cai
    • i-Lab, Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of Sciences
  • Feng Li
    • i-Lab, Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of Sciences
  • Yan Zhang
    • i-Lab, Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of Sciences
    • i-Lab, Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of Sciences
Open AccessResearch Article

DOI: 10.1007/s12274-010-0016-6

Cite this article as:
Cai, M., Li, F., Zhang, Y. et al. Nano Res. (2010) 3: 557. doi:10.1007/s12274-010-0016-6

Abstract

We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid (DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing.

Keywords

deoxyribonucleic acid (DNA) detection polymerase chain reaction (PCR) Au nanoparticle colorimetric

Copyright information

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2010