Research Article

Nano Research

, Volume 3, Issue 8, pp 557-563

Open Access This content is freely available online to anyone, anywhere at any time.

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

  • Miao CaiAffiliated withi-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences
  • , Feng LiAffiliated withi-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences
  • , Yan ZhangAffiliated withi-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences
  • , Qiangbin WangAffiliated withi-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences Email author 

Abstract

We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid (DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing.

Keywords

deoxyribonucleic acid (DNA) detection polymerase chain reaction (PCR) Au nanoparticle colorimetric