Research Article

Nano Research

, Volume 2, Issue 7, pp 543-552

Open Access This content is freely available online to anyone, anywhere at any time.

Direct imaging of titania nanotubes located in mouse neural stem cell nuclei

  • Yanli WangAffiliated withInstitute of Nanochemistry and Nanobiology, Shanghai University
  • , Jia WangAffiliated withInstitute of Nanochemistry and Nanobiology, Shanghai University
  • , Xiaoyong DengAffiliated withInstitute of Nanochemistry and Nanobiology, Shanghai University
  • , Jiao WangAffiliated withInstitute of Systems Biology, Shanghai University
  • , Haifang WangAffiliated withInstitute of Nanochemistry and Nanobiology, Shanghai University
  • , Minghong WuAffiliated withInstitute of Nanochemistry and Nanobiology, Shanghai University
  • , Zheng JiaoAffiliated withInstitute of Nanochemistry and Nanobiology, Shanghai University Email author 
  • , Yuanfang LiuAffiliated withInstitute of Nanochemistry and Nanobiology, Shanghai UniversityBeijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University Email author 

Abstract

Titania nanotubes (TiO2-NTs) are a potential drug vehicle for use in nanomedicine. To this end, a preliminary study of the interaction of a model cell with TiO2-NTs has been carried out. TiO2-NTs were first conjugated with a fluorescent label, fluorescein isothiocyanate (FITC). FITC-conjugated titania nanotubes (FITC-TiO2-NTs) internalized in mouse neural stem cells (NSCs, line C17.2) can be directly imaged by confocal microscopy. The confocal imaging showed that FITC-TiO2-NTs readily entered into the cells. After co-incubation with cells for 24 h, FITC-TiO2-NTs localized around the cell nucleus without crossing the karyotheca. More interestingly, the nanotubes passed through the karyotheca entering the cell nucleus after co-incubation for 48 h. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) were also employed in tracking the nanotubes in the cell. These results will be of benefit in future studies of TiO2-NTs for use as a drug vehicle, particularly for DNA-targeting drugs.

http://static-content.springer.com/image/art%3A10.1007%2Fs12274-009-9052-5/MediaObjects/12274_2009_9052_Fig1_HTML.jpg

Keywords

Titania nanotubes mouse neural stem cells nucleus confocal imaging atomic force microscopy