Research Article

Nano Research

, Volume 2, Issue 5, pp 365-372

Open Access This content is freely available online to anyone, anywhere at any time.

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

  • Xiaoliang WangAffiliated withNano Biomedicine Research Center, Med-X Research Institute, Shanghai Jiaotong University
  • , Linzhu ZhouAffiliated withNano Biomedicine Research Center, Med-X Research Institute, Shanghai Jiaotong University
  • , Yongjie MaAffiliated withNano Biomedicine Research Center, Med-X Research Institute, Shanghai Jiaotong University
  • , Xu LiAffiliated withNano Biomedicine Research Center, Med-X Research Institute, Shanghai Jiaotong University
  • , Hongchen GuAffiliated withNano Biomedicine Research Center, Med-X Research Institute, Shanghai Jiaotong University Email author 

Abstract

Using magnetic nanoparticles to enhance gene transfection, a recently developed technique termed magnetofection, has been shown to be a powerful technology in gene delivery. The most widely used magnetic nanoparticles in this area are those coated with polyethyleneimine, which is a well known nonviral transfection agent. In this article, we report methods to control the aggregate size of polyethyleneimine-coated magnetite particles. These particles were then used to enhance transfection of green fluorescent protein (GFP) into NIH 3T3 cells in vitro. We find that the aggregate size of the particles has a great effect on their performance in magnetofection, with less aggregated magnetic particles being more effective in enhancing the gene transfection.

http://static-content.springer.com/image/art%3A10.1007%2Fs12274-009-9035-6/MediaObjects/12274_2009_9035_Fig1_HTML.jpg

Keywords

Magnetofection transfection magnetic nanoparticles polyethyleneimine (PEI)