Open Access
Research Article

Nano Research

, Volume 2, Issue 5, pp 365-372

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

Authors

  • Xiaoliang Wang
    • Nano Biomedicine Research Center, Med-X Research InstituteShanghai Jiaotong University
  • Linzhu Zhou
    • Nano Biomedicine Research Center, Med-X Research InstituteShanghai Jiaotong University
  • Yongjie Ma
    • Nano Biomedicine Research Center, Med-X Research InstituteShanghai Jiaotong University
  • Xu Li
    • Nano Biomedicine Research Center, Med-X Research InstituteShanghai Jiaotong University
  • Hongchen Gu
    • Nano Biomedicine Research Center, Med-X Research InstituteShanghai Jiaotong University

DOI: 10.1007/s12274-009-9035-6

Abstract

Using magnetic nanoparticles to enhance gene transfection, a recently developed technique termed magnetofection, has been shown to be a powerful technology in gene delivery. The most widely used magnetic nanoparticles in this area are those coated with polyethyleneimine, which is a well known nonviral transfection agent. In this article, we report methods to control the aggregate size of polyethyleneimine-coated magnetite particles. These particles were then used to enhance transfection of green fluorescent protein (GFP) into NIH 3T3 cells in vitro. We find that the aggregate size of the particles has a great effect on their performance in magnetofection, with less aggregated magnetic particles being more effective in enhancing the gene transfection.

https://static-content.springer.com/image/art%3A10.1007%2Fs12274-009-9035-6/MediaObjects/12274_2009_9035_Fig1_HTML.jpg

Keywords

Magnetofection transfection magnetic nanoparticles polyethyleneimine (PEI)

Supplementary material

12274_2009_9035_MOESM1_ESM.pdf (381 kb)
Supplementary material, approximately 340 KB.

Copyright information

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2009