Nano Research

, Volume 2, Issue 3, pp 210–219

Myoglobin/gold nanoparticles/carbon spheres 3-D architecture for the fabrication of a novel biosensor

Authors

  • Xiao Chen
    • Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education, School of Chemistry and Chemical EngineeringNanjing University
  • Jing Jing Zhang
    • Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education, School of Chemistry and Chemical EngineeringNanjing University
  • Jie Xuan
    • Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education, School of Chemistry and Chemical EngineeringNanjing University
    • Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education, School of Chemistry and Chemical EngineeringNanjing University
Open AccessResearch Article

DOI: 10.1007/s12274-009-9019-6

Cite this article as:
Chen, X., Zhang, J.J., Xuan, J. et al. Nano Res. (2009) 2: 210. doi:10.1007/s12274-009-9019-6

Abstract

A novel biosensor based on a myoglobin/gold nanoparticles/carbon spheres (Mb-AuNPs-CNs) 3-D architecture bioconjunction has been fabricated for the determination of hydrogen peroxide (H2O2). Cyclic voltammetry (CV), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) were used to characterize the bioconjunction of the AuNPs-CNs with Mb. Experimental results demonstrate that the AuNPs-CNs hybrid material is more effective in facilitating electron transfer of the immobilized enzyme than CNs alone, which can be attributed to the unique nanostructure and larger surface area of the bioconjunction. The biosensor displayed good performance for the detection of H2O2 with a wide linear range from 0.28 μmol/L to 116.5 μmol/L and a detection limit of 0.12 μmol/L. The Michaelis-Menten constant KMapp value was estimated to be 0.3 mmol/L. The resulting biosensor exhibited fast amperometric response, and good stability, reproducibility, and selectivity to H2O2.

https://static-content.springer.com/image/art%3A10.1007%2Fs12274-009-9019-6/MediaObjects/12274_2009_9019_Fig1_HTML.gif

Keywords

Biosensormyoglobingold nanoparticlescarbon sphereshydrogen peroxidedirect electron transfer
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© Tsinghua University Press and Springer-Verlag GmbH 2009