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The isolation and the characterization of two polysaccharides from the branch bark of mulberry (Morus alba L.)

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Abstract

Two water-soluble polysaccharides termed MBBP-1 and MBBP-2 were isolated from the branches of the mulberry tree (Morus alba L.) using hot water extraction and purified on Anion-exchange DEAE52-cellulose and Sephadex G-100 column. MBBP-1 was shown to be composed of rhamnose, xylose, arabinose, mannose, glucose and galactose in the molar ratio of 4.53:2.49:4.38:4.67:17.85:5.88. MBBP-2 was composed of rhamnose, xylose, arabinose, mannose, glucose, galactose and galacturonic acid in the molar ratio of 26.85:13.8:3.14:4.4:6.1:3.19:4.9. Their structural characteristics were further investigated by FI-IR spectroscopy, Smith degradation, methylation analysis and NMR spectroscopy. Based on the data obtained, MBBP-1 had a backbone mainly consisting of (1 → 3)-linked glucose. MBBP-2 had a backbone mainly consisting of (1 → 3)-linked rhamnose and (1 → 2, 4)-linked xylose. Antioxidant assays indicated that antioxidant activities of MBBP-2 were significantly stronger than those of MBBP-1, and this was likely in relation to the different content of 8.2 % galacturonic acid in MBBP-2.

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Acknowledgments

The authors gratefully acknowledge the earmarked fund (CARS-22-ZJ0504) from China Agriculture Research System (CARS) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, P. R. China.

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Authors and Affiliations

  1. Applied Biological Department, Medical College of Soochow University, Suzhou, 215123, People’s Republic of China

    Fan Qiu, Tian-Zhen He & Yu-Qing Zhang

  2. The State Engineering Laboratory of Modern Silk, Soochow University, Suzhou, 215123, People’s Republic of China

    Fan Qiu, Tian-Zhen He & Yu-Qing Zhang

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  1. Fan Qiu
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Correspondence to Yu-Qing Zhang.

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Qiu, F., He, TZ. & Zhang, YQ. The isolation and the characterization of two polysaccharides from the branch bark of mulberry (Morus alba L.). Arch. Pharm. Res. 39, 887–896 (2016). https://doi.org/10.1007/s12272-016-0742-8

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