Research Articles Drug Actions

Archives of Pharmacal Research

, Volume 33, Issue 8, pp 1261-1267

An experimental study of rabbit conjunctival epithelial toxicity using co-treatment with Mitomycin-C and a histone deacetylase inhibitor

  • Tae-Hyun KimAffiliated withDepartment Anatomy and Cell Biology, Dong-A University College of Medicine and Medical Science Research CenterInstitute of Tissue Regeneration Engineering (ITREN), Dankook University
  • , Hyun Cheol OhAffiliated withDepartment of Ophthalmology, Dong-A University College of Medicine
  • , Young Hyun YooAffiliated withDepartment Anatomy and Cell Biology, Dong-A University College of Medicine and Medical Science Research Center
  • , Jee Hyun RhoAffiliated withDepartment of Ophthalmology, Dong-A University College of Medicine
  • , Woo Chan ParkAffiliated withDepartment of Ophthalmology, Dong-A University College of Medicine
  • , Hee Bae AhnAffiliated withDepartment of Ophthalmology, Dong-A University College of Medicine
  • , Woo Jin JeungAffiliated withDepartment of Ophthalmology, Dong-A University College of Medicine
  • , Sae Heun RhoAffiliated withDepartment of Ophthalmology, Dong-A University College of Medicine Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

This study was conducted to evaluate cytotoxicity due to co-treatment with low-dose Mitomycin-C (MMC) and the histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA) for glaucoma filtration surgery. In this study, the effect of co-treatment with MMC and SAHA to induce apoptosis in cultured conjunctival epithelial cells (CEs) in rabbit was investigated. The cytotoxic potential following co-treatment with MMC and SAHA in CEs via assay for reactive oxygen species (ROS) and lactate dehydrogenase (LDH) was also examined. Co-treatment with MMC and SAHA did not induce apoptosis in CEs. In addition, co-treatment with MMC and SAHA caused no significant alteration of ROS and LDH levels in CEs. This study therefore shows that low-dose MMC could still be used as an antimetabolite in co-treatment with SAHA to effectively inhibit fibrosis of Tenon’s capsule fibroblasts (TCFs) following glaucoma filtration surgery, while minimizing cytotoxicity in CEs.

Key words

Mitomycin-C Histone deacetylase inhibitor Suberoylanilide hydroxamic acid (SAHA) Apoptosis Glaucoma filtration surgery