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An experimental study of rabbit conjunctival epithelial toxicity using co-treatment with Mitomycin-C and a histone deacetylase inhibitor

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Abstract

This study was conducted to evaluate cytotoxicity due to co-treatment with low-dose Mitomycin-C (MMC) and the histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA) for glaucoma filtration surgery. In this study, the effect of co-treatment with MMC and SAHA to induce apoptosis in cultured conjunctival epithelial cells (CEs) in rabbit was investigated. The cytotoxic potential following co-treatment with MMC and SAHA in CEs via assay for reactive oxygen species (ROS) and lactate dehydrogenase (LDH) was also examined. Co-treatment with MMC and SAHA did not induce apoptosis in CEs. In addition, co-treatment with MMC and SAHA caused no significant alteration of ROS and LDH levels in CEs. This study therefore shows that low-dose MMC could still be used as an antimetabolite in co-treatment with SAHA to effectively inhibit fibrosis of Tenon’s capsule fibroblasts (TCFs) following glaucoma filtration surgery, while minimizing cytotoxicity in CEs.

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Authors and Affiliations

  1. Department Anatomy and Cell Biology, Dong-A University College of Medicine and Medical Science Research Center, Busan, 602-714, Korea

    Tae-Hyun Kim & Young Hyun Yoo

  2. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, Korea

    Tae-Hyun Kim

  3. Department of Ophthalmology, Dong-A University College of Medicine, Busan, 602-714, Korea

    Hyun Cheol Oh, Jee Hyun Rho, Woo Chan Park, Hee Bae Ahn, Woo Jin Jeung & Sae Heun Rho

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  1. Tae-Hyun Kim
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  2. Hyun Cheol Oh
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  3. Young Hyun Yoo
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Correspondence to Sae Heun Rho.

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Kim, TH., Oh, H.C., Yoo, Y.H. et al. An experimental study of rabbit conjunctival epithelial toxicity using co-treatment with Mitomycin-C and a histone deacetylase inhibitor. Arch. Pharm. Res. 33, 1261–1267 (2010). https://doi.org/10.1007/s12272-010-0817-x

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  • DOI: https://doi.org/10.1007/s12272-010-0817-x

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