Abstract
Membrane proteins are highly relevant for pharmaceutical research, but their biophysical properties pose tremendous difficulties for the preparation of high quality samples. Nanodiscs provide versatile tools to stabilize membrane proteins in defined phospholipid bilayers. A screening platform based on cell-free biosynthesis allows to identify ideal nanodisc/lipid combinations for the efficient folding, stabilization, and functional characterization of a large diversity of membrane proteins.
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Bernhard, F., Henrich, E. & Maertens, B. Funktionale Membranproteine durch optimierte Lipidumgebung in Nanodiscs. Biospektrum 21, 640–642 (2015). https://doi.org/10.1007/s12268-015-0626-3
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DOI: https://doi.org/10.1007/s12268-015-0626-3