Abstract
Heme oxygenase-1 has been identified to protect allograft from ischemia/reperfusion and immunologic rejection. Activity of heme oxygenase-1 is regulated by a guanine-thymine dinucleotide length polymorphism in the heme oxygenase-1 gene promoter. In this study, we aimed to explore the impact of the heme oxygenase-1 gene promoter polymorphism of donors and recipients on the orthotopic liver graft function after transplantation. Sixty recipients and their accompanying donors of orthotopic liver allografts were included retrospectively in this study. Heme oxygenase-1 gene promoter polymorphism was assessed using genomic DNA isolated from cryopreserved splenocytes or peripheral blood mononuclear cells and analyzed by genetic analyzer. Small allele of the donor heme oxygenase-1 gene polymorphism significantly prolonged the graft survival (p = 0.017). Recipients of allografts from a class of small-allele carrier had significantly lower serum total bilirubin compared with recipients of a nonclass small-allele donor liver (p < 0.01). Additionally, in recipients of small-carrier allografts, cold ischemia time (<10 h or ≥10 h) did not affect the total bilirubin significantly. Our study suggested a protective function of donor-derived heme oxygenase-1 gene promoter polymorphism on orthotopic liver allograft function after transplantation.
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Grant Information
This work was supported by grants from the National Natural Science Foundations of China (81170721), Shanghai Pujiang Program (14PJ1407300), and Medical Guiding Project of Shanghai Municipal Science and Education Commission (14411960700).
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This study protocol conforms to the ethical guidelines of the 1975 Declaration of Helsinki as reflected in a prior approval by the institution’s human research committee of Shanghai Jiao Tong University, and informed consent was obtained from each patient included in the study.
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The authors declare that they have no competing interests.
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Zheng-Yun Zhang and Jiao Guan contributed equally to this work.
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Zhang, ZY., Guan, J., Li, H. et al. Heme Oxygenase-1 Promoter Polymorphism Protects Liver Allograft. Indian J Surg 78, 14–19 (2016). https://doi.org/10.1007/s12262-015-1309-5
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DOI: https://doi.org/10.1007/s12262-015-1309-5