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Radiation-induced biomimetic modification of dual-layered nano/microfibrous scaffolds for vascular tissue engineering

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Abstract

One of the interesting strategies for developing the artificial blood vessels is to generate multi-layered scaffolds for mimicking the structure of native blood vessels such as the intima, media, and adventitia. In this study, we prepared dual-layered poly(L-lactide-co-ɛ-caprolactone) (PLCL) scaffolds with micro- and nanofibers as a basic construct of the vessel using electrospinning methods, which was functionalized using a gelatin through acrylic acid (AAc) grafting by γ-ray irradiation. Based on the microfibrous platform (fiber diameter 5 μm), the thickness of the nanofibrous layer (fiber diameter 700 nm) was controlled from 1.1 ± 0.8 to 32.2 ± 1.7 μm, and the mechanical property of the scaffolds was almost maintained despite the increase in thickness of the nanofibrous layer. The successful AAc graft by γ-ray irradiation could allow the gelatin immobilization on the scaffolds. The proliferation of smooth muscle cells (SMC) on the scaffolds toward a microfibrous layer was approximately 1.3-times greater than in the other groups, and the infiltration was significantly increased, presenting a wide cell distribution in the cross-section. In addition, human umbilical vein endothelial cell (HUVEC) adhesion toward nanofibrous layer was well-managed over the entire surface, and the accelerated proliferation was observed on the gelatin-functionalized scaffolds presenting the well-organized gap-junctions. Therefore, our biomimetic dual-layered scaffolds may be the alternative tools for replacing the damaged blood vessels.

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  1. Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Korea

    Young Min Shin, Jong-Young Lim, Jong-Seok Park, Hui-Jeong Gwon, Sung In Jeong & Youn-Mook Lim

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  1. Young Min Shin
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  2. Jong-Young Lim
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  3. Jong-Seok Park
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  4. Hui-Jeong Gwon
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  5. Sung In Jeong
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  6. Youn-Mook Lim
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Correspondence to Youn-Mook Lim.

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Shin, Y.M., Lim, JY., Park, JS. et al. Radiation-induced biomimetic modification of dual-layered nano/microfibrous scaffolds for vascular tissue engineering. Biotechnol Bioproc E 19, 118–125 (2014). https://doi.org/10.1007/s12257-013-0723-4

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  • DOI: https://doi.org/10.1007/s12257-013-0723-4

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