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Effects of Thermobifida fusca cutinase-carbohydrate-binding module fusion proteins on cotton bioscouring

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Abstract

Previously, we presented a novel approach for increasing Thermobifida fusca cutinase adsorption on cotton fibers by fusing cutinase with a carbohydrate-binding module (CBM). A preliminary study showed that two fusion proteins, namely cutinase-CBMCel6A and cutinase-CBMCenA, with similar stabilities and catalytic properties, had potential applications in bioscouring. In the present study, an indepth analysis of both cutinase-CBMs in bioscouring was explored. Effects of cutinase-CBMs on cotton bioscouring were investigated by characterizing the chemical and physical surface changes in enzyme-treated cotton fabrics. Gas chromatography/mass spectrometry was used to analyze the degradation of the cotton fabric cuticle; Fourier transform infrared microspectroscopy was used to study changes in the chemical composition of the cotton fabric epidermal layer; and scanning electron microscopy was used to monitor minor changes in the morphology of the fiber surface. Our results indicated that cutinase-CBMs in combination with pectinase had a greater effect on cotton fabric than did cutinase. Following scouring with cutinase-CBMs and pectinase, the performance of cotton fabric in terms of its wettability and dyeability was similar to that following alkali scouring. Our study provides a foundation for the further application of cutinase-CBM to bioscouring.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214-122, China

    Yao Zhang, Sheng Chen, Jing Wu & Jian Chen

  2. School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, Wuxi, 214-122, China

    Yao Zhang, Sheng Chen, Jing Wu & Jian Chen

  3. Honor School, Jiangnan University, Wuxi, 214-122, China

    Miao He

  4. School of Textile and Clothing, Jiangnan University, Wuxi, 214-122, China

    Qiang Wang

Authors
  1. Yao Zhang
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  2. Sheng Chen
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  3. Miao He
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  4. Jing Wu
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  5. Jian Chen
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  6. Qiang Wang
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Correspondence to Jing Wu.

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Zhang, Y., Chen, S., He, M. et al. Effects of Thermobifida fusca cutinase-carbohydrate-binding module fusion proteins on cotton bioscouring. Biotechnol Bioproc E 16, 645–653 (2011). https://doi.org/10.1007/s12257-011-0036-4

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  • DOI: https://doi.org/10.1007/s12257-011-0036-4

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