Virologica Sinica

, Volume 32, Issue 2, pp 147–154

Genome analysis of Heliothis virescens ascovirus 3h isolated from China

  • Guo-Hua Huang
  • Dian-Hai Hou
  • Manli Wang
  • Xiao-Wen Cheng
  • Zhihong Hu
Research Article

DOI: 10.1007/s12250-016-3929-8

Cite this article as:
Huang, GH., Hou, DH., Wang, M. et al. Virol. Sin. (2017) 32: 147. doi:10.1007/s12250-016-3929-8

Abstract

No ascovirus isolated from China has been sequenced so far. Therefore, in this study, we aimed to sequence the genome of Heliothis virescens ascovirus 3h (HvAV-3h) using the 454 pyrosequencing technology. The genome was found to be 190,519-bp long with a G+C content of 45.5%. We also found that it encodes 185 hypothetical open reading frames (ORFs) along with at least 50 amino acids, including 181 ORFs found in other ascoviruses and 4 unique ORFs. Gene-parity plots and phylogenetic analysis revealed a close relationship between HvAV-3h and three other HvAV-3a strains and a distant relationship with Spodoptera frugiperda ascovirus 1a (SfAV-1a), Trichoplusia ni ascovirus 6a (TnAV-6a), and Diadromus pulchellus ascovirus 4a (DpAV-4a). Among the 185 potential genes encoded by the genome, 44 core genes were found in all the sequenced ascoviruses. In addition, 25 genes were found to be conserved in all ascoviruses except DpAV-4a. In the HvAV-3h genome, 24 baculovirus repeat ORFs (bros) were present, and the typical homologous repeat regions (hrs) were absent. This study supplies information important for understanding the conservation and functions of ascovirus genes as well as the variety of ascoviral genomes.

Keywords

Heliothis virescens ascovirus 3h (HvAV-3h) genome organization bro genes phylogenetic relationship 

Supplementary material

12250_2016_3929_MOESM1_ESM.pdf (917 kb)
Genome analysis of Heliothis virescens ascovirus 3h isolated from China

Copyright information

© Wuhan Institute of Virology, CAS and Springer Science+Business Media Singapore 2017

Authors and Affiliations

  • Guo-Hua Huang
    • 1
    • 2
  • Dian-Hai Hou
    • 1
  • Manli Wang
    • 1
  • Xiao-Wen Cheng
    • 3
  • Zhihong Hu
    • 1
  1. 1.State Key Laboratory of Virology, Microorganisms & Viruses Culture Collection Center, Wuhan Institute of VirologyChinese Academy of SciencesWuhan, HubeiChina
  2. 2.Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Institute of VirologyHunan Agricultural UniversityChangsha, HunanChina
  3. 3.Department of Microbiology, 32 Pearson HallMiami UniversityOxfordUSA

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