Virologica Sinica

, Volume 31, Issue 5, pp 406–414

Ficolin-2 binds to HIV-1 gp120 and blocks viral infection

Research Article

DOI: 10.1007/s12250-016-3808-3

Cite this article as:
Luo, F., Chen, T., Liu, J. et al. Virol. Sin. (2016) 31: 406. doi:10.1007/s12250-016-3808-3
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Abstract

Ficolin-2 is a lectin complement pathway activator present in normal human plasma and usually associated with infectious diseases, but little is known about the role of ficolin-2 in human immunodeficiency virus (HIV) infection. Here, we describe our novel findings that serum ficolin-2 concentrations of 103 HIV-1 patients were much higher compared to those of 57 healthy donors. In vitro analysis showed that HIV-1 infection could enhance ficolin-2 expression. We further demonstrated that recombinant ficolin-2 protein could bind with HIV-1 envelope glycoprotein gp120, and subsequently induce complement dependent cytotoxicity. Moreover, ficolin-2 could block the entry of HIV-1 into target cells (TZM-b1 and MT-2 cells) and infection in a ficolin-2 dosedependent manner. To our knowledge, this is the first report about the protective role of ficolin-2 against HIV-1 infection and our study suggests that ficolin-2 is an important human innate immune molecule against HIV.

Keywords

ficolin-2 HIV-1 gp120 

Copyright information

© Wuhan Institute of Virology, CAS and Springer Science+Business Media Singapore 2016

Authors and Affiliations

  1. 1.The State Key Laboratory of Virology, Department of Immunology and Hubei Province Key Laboratory of Allergy and Immune-related DiseasesWuhan University School of MedicineWuhanChina
  2. 2.Zhongnan Hospital of Wuhan UniversityWuhanChina
  3. 3.Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina