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A respiratory syncytial virus persistent-infected cell line system reveals the involvement of SOCS1 in the innate antiviral response

  • Research Article
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Virologica Sinica

Abstract

HEp-2 cells persistently infected with respiratory syncytial virus (RSV) are a heterogeneous mixture of viral antigen-positive and -negative variants; however, the mechanism through which viral replication becomes latent remains unclear. In this study, we investigated the potential mechanism by which RSV escapes from innate immune surveillance. Persistent-infected RSV HEp-2 cells were isolated and cell clones were passaged. The RSV-persistent cells produced viruses at a lower titer, resisted wild-type RSV re-infection, and secreted high levels of interferon-ß (IFN-ß), macrophage inflammatory protein-1α (Mip-1α), interleukin-8 (IL-8), and Rantes. Toll-like receptor 3 (TLR3), retinoic acid inducible gene-I (RIG-I), and suppressor of cytokine signaling 1 (SOCS1) levels were upregulated in these cells. The silencing of TLR3 mRNA decreased the expression of SOCS1 protein and the secretion of cytokines. RSV-persistent cells are in an inflammatory state; upregulation of SOCS1 is related to the TLR3 signaling pathway, which could be associated with the mechanism of viral persistence.

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Authors and Affiliations

  1. Department of Pediatrics, Zhongnan Hospital of Wuhan University, Donghu Road 169, Wuhan, 430071, China

    Junwen Zheng, Pu Yang & Dongchi Zhao

  2. Department of Anatomy, School of Medicine, Wuhan University, Donghu Road 185, Wuhan, 430071, China

    Yan Tang

Authors
  1. Junwen Zheng
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  2. Pu Yang
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  3. Yan Tang
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  4. Dongchi Zhao
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Correspondence to Dongchi Zhao.

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ORCID: 0000-0002-9454-6041

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Zheng, J., Yang, P., Tang, Y. et al. A respiratory syncytial virus persistent-infected cell line system reveals the involvement of SOCS1 in the innate antiviral response. Virol. Sin. 30, 190–199 (2015). https://doi.org/10.1007/s12250-015-3597-0

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  • DOI: https://doi.org/10.1007/s12250-015-3597-0

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