Research Article

Virologica Sinica

, Volume 29, Issue 1, pp 33-39

Calcium phosphate nanoparticles show an effective activation of the innate immune response in vitro and in vivo after functionalization with flagellin

  • Diana KozlovaAffiliated withInstitute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen
  • , Viktoriya SokolovaAffiliated withInstitute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen
  • , Maohua ZhongAffiliated withMucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
  • , Ejuan ZhangAffiliated withMucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
  • , Jingyi YangAffiliated withMucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
  • , Wei LiAffiliated withMucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
  • , Yi YangAffiliated withMucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
  • , Jan BuerAffiliated withInstitute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen
  • , Astrid Maria WestendorfAffiliated withInstitute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen
    • , Matthias EppleAffiliated withInstitute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen Email author 
    • , Huimin YanAffiliated withMucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences Email author 

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Abstract

For subunit vaccines, adjuvants play a key role in shaping the magnitude, persistence and form of targeted antigen-specific immune response. Flagellin is a potent immune activator by bridging innate inflammatory responses and adaptive immunity and an adjuvant candidate for clinical application. Calcium phosphate nanoparticles are efficient carriers for different biomolecules like DNA, RNA, peptides and proteins. Flagellin-functionalized calcium phosphate nanoparticles were prepared and their immunostimulatory effect on the innate immune system, i.e. the cytokine production, was studied. They induced the production of the proinflammatory cytokines IL-8 (Caco-2 cells) and IL-1β (bone marrow-derived macrophages; BMDM) in vitro and IL-6 in vivo after intraperitoneal injection in mice. The immunostimulation was more pronounced than with free flagellin.

Keywords

calcium phosphate nanoparticle immunostimulation innate immune system flagellin adjuvants