Virologica Sinica

, Volume 29, Issue 1, pp 33–39

Calcium phosphate nanoparticles show an effective activation of the innate immune response in vitro and in vivo after functionalization with flagellin

Authors

  • Diana Kozlova
    • Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE)University of Duisburg-Essen
  • Viktoriya Sokolova
    • Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE)University of Duisburg-Essen
  • Maohua Zhong
    • Mucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of Sciences
  • Ejuan Zhang
    • Mucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of Sciences
  • Jingyi Yang
    • Mucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of Sciences
  • Wei Li
    • Mucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of Sciences
  • Yi Yang
    • Mucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of Sciences
  • Jan Buer
    • Institute of Medical Microbiology, University Hospital EssenUniversity of Duisburg-Essen
  • Astrid Maria Westendorf
    • Institute of Medical Microbiology, University Hospital EssenUniversity of Duisburg-Essen
    • Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE)University of Duisburg-Essen
    • Mucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of Sciences
Research Article

DOI: 10.1007/s12250-014-3379-0

Cite this article as:
Kozlova, D., Sokolova, V., Zhong, M. et al. Virol. Sin. (2014) 29: 33. doi:10.1007/s12250-014-3379-0

Abstract

For subunit vaccines, adjuvants play a key role in shaping the magnitude, persistence and form of targeted antigen-specific immune response. Flagellin is a potent immune activator by bridging innate inflammatory responses and adaptive immunity and an adjuvant candidate for clinical application. Calcium phosphate nanoparticles are efficient carriers for different biomolecules like DNA, RNA, peptides and proteins. Flagellin-functionalized calcium phosphate nanoparticles were prepared and their immunostimulatory effect on the innate immune system, i.e. the cytokine production, was studied. They induced the production of the proinflammatory cytokines IL-8 (Caco-2 cells) and IL-1β (bone marrow-derived macrophages; BMDM) in vitro and IL-6 in vivo after intraperitoneal injection in mice. The immunostimulation was more pronounced than with free flagellin.

Keywords

calcium phosphatenanoparticleimmunostimulationinnate immune systemflagellinadjuvants

Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2014