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A cryptic taxon rather than a hybrid species of Tragopogon (Asteraceae) from the Czech Republic

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Summary

Tragopogon ×mirabilis Rouy is described as a diploid hybrid between T. porrifolius and T. pratensis. A population of T. ×mirabilis from Central Bohemia, Czech Republic, was recently investigated and, unlike previous reports of T. ×mirabilis, was found to be highly fertile. This fertile diploid hybrid population was considered to represent an alternative evolutionary pathway to polyploidy in Tragopogon. To determine the parentage of the plants from Bohemia, we investigated 12 samples of T. ×mirabilis with ITS, ETS, LFY and plastid (rpL16 gene, intron 1, tRNA-Leu (trnL) gene, intron, trnL-trnF intergenic spacer, psbA-trnH intergenic spacer, and trnG-trnT intergenic spacer) sequence data. None of the Bohemian plants have sequences that are consistent with a hybrid origin between T. porrifolius (incl. T. australis) and T. pratensis. Our data suggest that this fertile population of “T. ×mirabilis” may represent an unrecognised diploid species from the Angustissimi clade sensu Mavrodiev et al. (Int. J. Pl. Sci. 164: 1 – 19, 2005), a clade with a centre of distribution in the Caucasus, and hybrids of this unknown species with T. orientalis or T. hayekii (= T. orientalis L. var. hayekii Soó), a species closely related to T. pratensis and native to Bohemia. The Bohemian population of “Tragopogon ×mirabilis” clearly requires more investigation, but based on our data it does not appear to represent T. porrifolius × T. pratensis.

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Acknowledgements

We thank Dr Alex A. Sennikov (University of Helsinki, Finland) for providing samples of leaf material. This work was support by US NSF grants MCB-0346437 and DEB-0614421.

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Authors and Affiliations

  1. Department of Biology, University of Florida, Gainesville, FL, 32611, USA

    Evgeny V. Mavrodiev & Douglas E. Soltis

  2. Florida Museum of Natural History, University of Florida, Gainesville, FL, 32611, USA

    Evgeny V. Mavrodiev, Douglas E. Soltis & Pamela S. Soltis

  3. Institute of Botany, Academy of Sciences of the Czech Republic, Prùhonice, CZ, 252 43, Czech Republic

    Frantisek Krahulec

Authors
  1. Evgeny V. Mavrodiev
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  2. Frantisek Krahulec
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  3. Douglas E. Soltis
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  4. Pamela S. Soltis
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Correspondence to Evgeny V. Mavrodiev.

Appendix 1

Appendix 1

Voucher data and the GenBank accession numbers for individuals sequenced in this study.

Tragopogon cretaceus S. A. Nikitin: European Russia, det. Tzvelev N. N. (MW). 5.8S rRNA gene, internal transcribed spacer 1 (ITS 1) and 2 (ITS 2): HQ456271; external transcribed spacer: HQ456289.

T. elatior Steven: Laspi, a mare ad altitudi 200 hexap., Taur. merid., Compere (H). Internal transcribed spacer 1 (ITS1): HQ456272.

T. ×mirabilis Rouy: Roudnice nad Labem, 14 – 15E, 50 – 25 N, alt. c. 180 – 190 m. 15 Oct. 2005, Jan Novak. Field collections. 5.8S rRNA gene, internal transcribed spacer 1 (ITS 1) and 2 (ITS 2): HQ456256–68; LEAFY (LFY) gene, intron 2: HQ456290–91; psbA-trnH intergenic spacer, plastid: HQ456292–93; rpL16 gene, intron 1: HQ456294–95; trnG-trnT intergenic spacer: HQ456296–97; trnL-trnF intergenic spacer: HQ456298–99; tRNA-Leu (trnL) gene, intron: HQ456300–01.

T. porrifolius L.: Slovenia, Botanic Garden, Ljubljana, collection # 1007. 5.8S rRNA gene, internal transcribed spacer 1 (ITS 1) and 2 (ITS 2): HQ456269; external transcribed spacer: HQ456287.

T. pratensis L.: Czech Republic, NW part, Cheb, between villages Uboci and Zandov, 50°02.375'N, 12°33.782'E, altitude 528 m, field collection # 929; cultivated in the garden of the Institute of Botany, Academy of Science of the Czech Republic, Pruhonice, Czech Republic, Nov. 2005, 5.8S rRNA gene, internal transcribed spacer 1 (ITS 1) and 2 (ITS 2): HQ456270; external transcribed spacer: HQ456288.

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Mavrodiev, E.V., Krahulec, F., Soltis, D.E. et al. A cryptic taxon rather than a hybrid species of Tragopogon (Asteraceae) from the Czech Republic. Kew Bull 68, 133–141 (2013). https://doi.org/10.1007/s12225-012-9431-z

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