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Bacterial metabolites from intra- and inter-species influencing thermotolerance: the case of Bacillus cereus and Geobacillus stearothermophilus

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Abstract

Bacterial metabolites with communicative functions could provide protection against stress conditions to members of the same species. Yet, information remains limited about protection provided by metabolites in Bacillus cereus and inter-species. This study investigated the effect of extracellular compounds derived from heat shocked (HS) and non-HS cultures of B. cereus and Geobacillus stearothermophilus on the thermotolerance of non-HS vegetative and sporulating B. cereus. Cultures of B. cereus and G. stearothermophilus were subjected to HS (42 or 65 °C respectively for 30 min) or non-HS treatments. Cells and supernatants were separated, mixed in a combined array, and then exposed to 50 °C for 60 min and viable cells determined. For spores, D values (85 and 95 °C) were evaluated after 120 h. In most cases, supernatants from HS B. cereus cultures added to non-HS B. cereus cells caused their thermotolerance to increase (D 50 12.2–51.9) when compared to supernatants from non-HS cultures (D 50 7.4–21.7). While the addition of supernatants from HS and non-HS G. stearothermophilus cultures caused the thermotolerance of non-HS cells from B. cereus to decrease initially (D 50 3.7–7.1), a subsequent increase was detected in most cases (D 50 18–97.7). In most cases, supernatants from sporulating G. stearothermophilus added to sporulating cells of B. cereus caused the thermotolerance of B. cereus 4810 spores to decline, whereas that of B. cereus 14579 increased. This study clearly shows that metabolites in supernatants from either the same or different species (such as G. stearothermophilus) influence the thermotolerance of B. cereus.

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Acknowledgements

This work was supported by a grant from the Consejo Nacional de Ciencia y Tecnología de México (CONACYT) grant no. 156073 and the Universidad Autónoma de Nuevo León. M.G. acknowledges a postgraduate fellowship from CONACYT.

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  1. Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Laboratorio de Bioquímica y Genética de Microorganismos, Ciudad Universitaria, 66455, San Nicolás de los Garza, N.L, Mexico

    Mayra Alejandra Gómez-Govea, Santos García & Norma Heredia

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  1. Mayra Alejandra Gómez-Govea
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  2. Santos García
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  3. Norma Heredia
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Correspondence to Norma Heredia.

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Gómez-Govea, M.A., García, S. & Heredia, N. Bacterial metabolites from intra- and inter-species influencing thermotolerance: the case of Bacillus cereus and Geobacillus stearothermophilus . Folia Microbiol 62, 183–189 (2017). https://doi.org/10.1007/s12223-016-0487-2

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