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Evaluation of antimicrobial resistance among Salmonella and Shigella isolates in the University Hospital “St. George,” Plovdiv, Bulgaria

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Abstract

The aim of this work is to study the epidemiology and antimicrobial resistance to the most commonly used antibiotics for the treatment of acute gastroenteritis caused by Salmonella and Shigella at the largest Bulgarian hospital—University Hospital “St. George,” Plovdiv—for the period 2009–2013. Two hundred ninety strains were in vitro tested for resistance to 15 antimicrobial agents. The presence of extended-spectrum beta-lactamases (ESBLs) was demonstrated by a variety of specialized tests. For comparison, a collection of 28 strains submitted by the National Reference Laboratory (NRL) “Enteric Infections” at the National Center of Infectious and Parasitic Diseases (NCIPD), Sofia, was also tested for the production of ESBLs. In isolates, phenotypically demonstrated as ESBL producers, polymerase chain reaction (PCR) detection of the genes bla-CTX-M, bla-SHV, and bla-TEM was performed. Among the 290 tested isolates, only two— Salmonella serotype Livingstone and Shigella flexneri—were phenotypically proven to be ESBL producers. Only 4 strains from the collection of 28, submitted from the NRL “Intestinal Infections” in NCIPD, Sofia, were phenotypically confirmed as ESBL producers. The presence of the bla-CTX-M gene was detected in all of the tested strains (4 from NRL, NCIPD, Sofia, and 2 from the University Hospital St. George, Plovdiv), the bla-SHV gene only in strain S. Livingstone from Plovdiv, and the bla-TEM gene in two from Sofia and one (again S. Livingstone) from Plovdiv. In conclusion, Salmonella and Shigella isolates from patients hospitalized at the University Hospital St. George, Plovdiv, with acute gastroenteritis demonstrate good susceptibility to the most commonly used antibiotic agents, including azithromycin.

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Acknowledgments

The authors express gratitude to the team of the National Referent Laboratory “Control and Monitoring of Antimicrobial Resistance” at the NCIPD, Sofia, for the submitted positive controls for PCR reactions.

The study is funded by research project no. 19/2013 of the Medical University of Plovdiv.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Pharmacy, Medical University-Plovdiv, 15A “Vassil Aprilov” Blvd, 4000, Plovdiv, Bulgaria

    Michael M. Petrov, Atanaska Petrova, Irina Stanimirova, Marina Mircheva-Topalova & Marianna Murdjeva

  2. Laboratory of Microbiology, University Hospital “St. George”-Plovdiv, 15A Vassil Aprilov Blvd, Plovdiv, Bulgaria

    Atanaska Petrova, Irina Stanimirova, Lalka Koycheva & Marianna Murdjeva

  3. Department of Infectious Diseases and Parasitology, Faculty of Medicine, Medical University-Plovdiv and University Hospital “St. George”-Plovdiv, Plovdiv, Bulgaria

    Rayna Velcheva & Mariana Stoycheva-Vartigova

  4. Department of Social Medicine and Public Health, Faculty of Public Health, Medical University-Plovdiv, 15A Vassil Aprilov Blvd, Plovdiv, Bulgaria

    Ralitsa Raycheva

  5. National Reference Laboratory “Enteric infections,” National Center of Infectious and Parasitic Diseases, 26 Yanko Sakazov Blvd., Sofia, Bulgaria

    Galina Asseva & Petar Petrov

  6. Department of Life Science, “Aquachim JSC”, 83 Prof Tzv. Lazarov Blvd., Sofia, Bulgaria

    Velichka Kardjeva

Authors
  1. Michael M. Petrov
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  2. Atanaska Petrova
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  3. Irina Stanimirova
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  4. Marina Mircheva-Topalova
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  5. Lalka Koycheva
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  6. Rayna Velcheva
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  7. Mariana Stoycheva-Vartigova
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  8. Ralitsa Raycheva
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  9. Galina Asseva
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  10. Petar Petrov
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  11. Velichka Kardjeva
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  12. Marianna Murdjeva
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Corresponding author

Correspondence to Michael M. Petrov.

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Petrov, M.M., Petrova, A., Stanimirova, I. et al. Evaluation of antimicrobial resistance among Salmonella and Shigella isolates in the University Hospital “St. George,” Plovdiv, Bulgaria. Folia Microbiol 62, 117–125 (2017). https://doi.org/10.1007/s12223-016-0478-3

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  • DOI: https://doi.org/10.1007/s12223-016-0478-3

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