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Anti-Outer membrane protein C antibodies in colorectal neoplasia

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Abstract

Sporadic colorectal cancer (CRC) represents an enormous problem worldwide. Large intestinal microbiota play an important role in the colorectal carcinogenesis. The aim of the study was to investigate anti-Outer membrane protein C (anti-OmpC) antibodies, aimed at porin C, which is embedded in the outer membrane of gram-negative bacteria, in patients with colorectal adenoma (CRA), CRC and controls. The study included 22 patients with CRA (11 men, 11 women, aged 26–79, mean 65 ± 12), 11 patients with CRC (9 men, 2 women, aged 50–83, mean 66 ± 11) and 45 controls, blood donors (24 men, 21 women, aged 20–58, mean 38 ± 10). Serum anti-OmpC antibodies were investigated by means of ELISA. Values of 0–20 U/mL were considered to be negative; values >25 U/mL were assessed as positive. A total of 9/11 (82 %) patients with CRC had positive anti-OmpC antibodies. Anti-OmpC antibodies were negative or grey-zone in 37/45 (82 %) controls. Serum anti-OmpC were found to be significantly higher in patients with CRC (median 42.4, interquartile range (IQR) 22.2) compared to controls (median 18.3, IQR 12.4), p < 0.001. No statistically significant difference in anti-OmpC was found between controls (median 18.3, IQR 12.4) and CRA patients (median 17.7, IQR 16.5), p = 0.326. Anti-OmpC were significantly higher in patients with CRC (median 42.4, IQR 22.2) compared to patients with CRA (median 17.7, IQR 16.5), p = 0.011. Positivity of anti-OmpC antibodies was found in patients with CRC, which supports the contribution of gram-negative large intestinal microbiota to the pathogenesis of CRC.

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Acknowledgments

The study was supported by research grant IGA NT/13413 from the Czech Ministry of Health.

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Authors and Affiliations

  1. 2nd Department of Internal Medicine - Gastroenterology, Charles University in Prague, Faculty of Medicine at Hradec Kralove, University Teaching Hospital, Sokolska 581, 50005, Hradec Kralove, Czech Republic

    D. Kohoutova, P. Moravkova & J. Bures

  2. Division of Surgery and Interventional Science, University College London, 67-73 Riding House Street, London, W1W 7EJ, UK

    D. Kohoutova

  3. Department of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine at Hradec Kralove, University Teaching Hospital, Sokolska 581, 50005, Hradec Kralove, Czech Republic

    M. Drahosova

  4. Department of Mathematics, Faculty of Science, University of Hradec Kralove, Jana Koziny 1237, 500 03, Hradec Kralove, Czech Republic

    M. Cihak

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  1. D. Kohoutova
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  2. M. Drahosova
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  4. P. Moravkova
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  5. J. Bures
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Correspondence to D. Kohoutova.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Kohoutova, D., Drahosova, M., Cihak, M. et al. Anti-Outer membrane protein C antibodies in colorectal neoplasia. Folia Microbiol 61, 295–299 (2016). https://doi.org/10.1007/s12223-015-0437-4

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  • DOI: https://doi.org/10.1007/s12223-015-0437-4

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