Abstract
The present study focuses on the detection of enterococci in ostrich faeces. Forty-six bacterial colonies from 140 ostriches were identified at the species level using the MALDI-TOF MS identification system. According to the score value evaluation, they were allotted to the species Enterococcus hirae, Enterococcus faecium and Enterococcus mundtii confirmed also by phenotypic testing. Dominated species E. hirae (34 strains) were submitted to more detailed testing. Those strains E. hirae produced either no or only slight amount of the enzymes related to disorders (N-acetyl-β-glucosaminidase, β-glucuronidase, α-chymotrypsin, trypsin). Most of the strains were not hemolytic. They did not harbour the hiracin-producing gene. Five E. hirae strains harboured virulence factor gene gelE; however, they were phenotypically gelatinase negative. They also harboured other virulence factor genes such as esp, efaAfm and ccf. E. hirae strains were mostly sensitive to antibiotics and those resistant at least to one antibiotic were sensitive to enterocins (200–25,600 AU/mL). This study represents original and novel results concerning the enterococcal microflora in ostriches; enterococci in ostriches have not been described in detail up to now; sensitivity to enterocins of E. hirae strains harbouring virulence factor genes to enterocins is also new.
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References
Alatoom AA, Cunningham SA, Ihde SM, Mandrekar J, Patel R (2011) Comparison of direct colony method versus extraction method for identification of Gram-positive cocci by use of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 49:2868–2873
Clinical and Laboratory Standards Institute- CLSI (2012) Performance Standards for Antimicrobial Disk Susceptibility tests. In: CLSI=NCCLS, Clinical and Laboratory Standards Institute, Wayne, PA, 27:M02-A11, Vol. 32, No. 1, M02-A10, Vol. 29, No. 1.
Cooper RG (2005) Bacterial, fungal and parasitic infections in the ostrich (Struthio camelus var. domesticus). Anim Sci J 76:97–106
De Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer K-H and Whitman WB. 2009. In: Bergeys Manual of Systematic Bacteriology, Second Eds. Springer, New York, Vol. 3, ISBN 0- 387- 95041–9.
De Vuyst L, Callewaert R, Pot B (1996) Characterization of antagonistic activity of Lactobacillus amylovorus DCE471 and large scale isolation of its bacteriocin amylovorin L471. Syst Appl Microbiol 19:9–20
Franz CHMAP, Stiles E, Schleifer KH, Holzapfel W (2003) Enterococci in foods-a conundrum for food safety. Int J Food Microbiol 88:105–122
Franz CHMAP, van Belkum M, Holzapfel W, Abriouel H, Gálvez A (2007) Diversity of enterococcal bacteriocins and their grouping in a new classification scheme. FEMS Microbiol Rev 31:293–310
Franz CHMAP, Huch M, Abriouel H, Holzapfel W, Gálvez A (2011) Enterococci as probiotics and their implications in food safety. Int J Food Microbiol 151:125–140
ISO −7899-detection and enumeration of intestinal enterococci. Part 2, http://www.sipe-rtd.inf/directive)
Jennes W, Dicks LMT, Wevoerd DJ (2000) Enterocin 012, a bacteriocin produced by Enterococcus gallinarum isolated from the intestinal tract of ostrich. J Appl Microbiol 88:349–357
Kandričáková A (2014) Aditive bacteria and their use for health (in Slovak) Dissertation-University of veterinary Medicine and Pharmacy-Institute of Animal Physiology Slovak Academy of Sciences. Košice, Slovakia, pp 1–182
Kondo H, Nariaki A, Tsukuda K, Wada Y (1997) Adherence of Enterococcus hirae to the duodenal epithelium of chicks with diarrhoea. Avian Pathol 26:189–194
Lauková A, Mareková M, Javorský P (1993) Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM 4231. Lett Appl Microbiol 16:257–260
Lauková A, Chrastinová Ľ, Pogány Simonová M, Strompfová V, Plachá I, Čobanová K, Formelová Z, Chrenková M, Ondruška Ľ (2012a) Enterococcus faecium AL AL41, its Enterocin M and their beneficial use in rabbits husbandry. Prob Antimicrob Prot 4:243–249
Lauková A, Hádryová J, Imrichová J, Strompfová V, Kandričáková A (2012b) Enterococus faecium EM41 isolate from ostrichs and its enterocin. Folia Vet 56:34–36
Lidbeck A, Nord CE, Gustafsson JA, Rafter J (1992) Lactobacilli, anticarcinogenic activities and human intestinal microflora. Eur J Can Prev 1:341–353
Mareková M, Lauková A, De Vuyst L, Skaugen M, Nes IF (2003) Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13. J Appl Microbiol 94:523–530
Mareková M, Lauková A, Skaugen M, Nes IF (2007) Isolation and characterization of a new bacteriocin, termed enterocin M, produced by environmental isolate Enterococcus faecium AL41. J Ind Microbiol Biotechnol 34:533–537
Paragraph 8, section 1 of Law No. 110/1972 Status related to animal breeding.
Pogány Simonová M, Lauková A, Chrastinová Ľ, Plachá I, Strompfová V, Čobanová K, Formelová Z, Chrenková M (2013) Combined administration of bacteriocin-producing, probiotic strain Enterococcus faecium CCM7420 with Eleutherococcus senticosus and their effect in rabbits. Polish J Vet Sci 16:619–627
Ribeiro T, Oliveira M, Fraqueza MJ, Lauková A, Elias M, Tenreiro R, Barreto AS, Semedo-Lemsaddek T (2011) Antibiotic resistance and virulence factors among enterococci isolated from chourico, a traditional Portuguese dry fermented sausage. J Food Prot 74:465–469
Sabolová G, Egyedová A, Gulovič J (2008) Ostrich as a farming animal (in Slovak). Slovak Vet J 32:21–27
Sánchez J, Borrero J, Gómez-Sala B, Basanta A, Herranz C, Cintas LM, Hérnandez PE (2008) Cloning and heterologous production of hiracin JM79, a sec-dependent bacteriocin produced by Enterococcus hirae DCH5 in lactic acid bacteria and Pichia pastoris. Appl Environ Microbiol 74:2471–2479
Semedo T, Santos MA, Lopes MFS, Figueiredo Marques JJ, Barreto Crespo MT, Tenreiro R (2003) Virulence factors in food, clinical and reference Enterococci: a common trait in the genus? Syst Appl Microbiol 26:13–22
Semedo-Lemsaddek T, Nóbrega CS, Ribeiro T, Pedroso NP, Sales-Luis T, Lemsaddek A, Tenreiro R, Tavares L, Vilela CL, Oliveira M (2013) Virulence traits and antibiotic resistance among enterococci isolated from Eurasian otter (Lutra lutra). Vet Microbiol 163:378–382
Strompfová V, Lauková A (2007) In vitro study on bacteriocin production of Enterococci associated with chickens. Anaerobe 13:228–237
Tejedor-Junco MT, Alfonso-Rodríguez O, Martín-Barrasa JL, González-Martín M (2005) Antimicrobial susceptibility of Enterococcus strains isolated from poultry faeces. Res Vet Sci 78:33–38
Van Wamel WJB, Hednrickx AP, Bonten MJM, Top J, Posthuma G, Willems RJ (2007) Growth condition-dependent esp expression by Enterococcus faecium affects initial adherence and biofilm formation. Infect Immun 75:924–931
Yoshimura H, Ishimaru M, Endoh YS, Kojima A (2000) Antimicrobial susceptibilities of enterococci isolated from faeces of broiler and layer chickens. Lett Appl Microbiol 31:427–432
Acknowledgments
These results were achieved in the framework of the projects Vega 2/0004/14 and Vega 2/0002/11. We are grateful to Mrs. Margita Bodnárová for her skillful assistance and to Dr. Teresa Semedo-Lemsaddek (Faculty of Veterinary Medicine in Lisbon) for her supervision at the detection of virulence factor genes during Dr. Strompfová’s study stay at Lisbon. Part of the preliminary results was presented at conference by Kandričáková A. et al. as Short communication-Folia Veterinaria 56, Suppl. II, 2012: 33–34.
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Lauková, A., Kandričáková, A., Ščerbová, J. et al. Enterococci isolated from farm ostriches and their relation to enterocins. Folia Microbiol 61, 275–281 (2016). https://doi.org/10.1007/s12223-015-0435-6
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DOI: https://doi.org/10.1007/s12223-015-0435-6