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Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.

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Abstract

The genus Aeromonas comprises primarily aquatic bacteria and also serious human and animal pathogens with the occurrence in clinical material, drinking water, and food. Aeromonads are typical for their complex taxonomy and nomenclature and for limited possibilities of identification to the species level. According to studies describing the use of MALDI-TOF MS in diagnostics of aeromonads, this modern chemotaxonomical approach reveals quite high percentage of correctly identified isolates. We analyzed 64 Aeromonas reference strains from the set of 27 species. After extending the range of analyzed Aeromonas species by newly described ones, we proved that MALDI-TOF MS procedure accompanied by Biotyper tool is not a reliable diagnostic technique for aeromonads. We obtained quite high percentage of false-positive, incorrect, and uncertain results. The identification of newly described species is accompanied with misidentifications that were observed also in the case of pathogenic aeromonads.

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Acknowledgments

This work was supported by project CEITEC—Central European Institute of Technology (CZ.1.05/1.1.00/02.0068)—and CEB—Center of Experimental Biomedicine (CZ.1.07/2.3.00/20.0183)—and funded by the European Regional Development Fund, by the Ministry of Agriculture of the Czech Republic (QI101A094 and MZE0002716202), and by Student Project Grant at Masaryk University (specific research, rector’s program) MUNI/A/0884/2013.

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Authors and Affiliations

  1. Division of Microbiology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 735/5, 625 00, Brno, Czech Republic

    Andrea Vávrová, Tereza Balážová & Ludmila Tvrzová

  2. Research Group Proteomics, CEITEC, Central European Institute of Technology, Masaryk University, Kamenice 735/5, 625 00, Brno, Czech Republic

    Tereza Balážová & Ondrej Šedo

  3. Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 735/5, 625 00, Brno, Czech Republic

    Ivo Sedláček

  4. National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 735/5, 625 00, Brno, Czech Republic

    Ondrej Šedo

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  1. Andrea Vávrová
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  2. Tereza Balážová
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Correspondence to Andrea Vávrová.

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Vávrová, A., Balážová, T., Sedláček, I. et al. Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.. Folia Microbiol 60, 375–383 (2015). https://doi.org/10.1007/s12223-014-0369-4

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