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Characterization of five newly isolated bacteriophages active against Pseudomonas aeruginosa clinical strains

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Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections, especially in patients with immunodeficiency. It exhibits multiple mechanisms of resistance, including efflux pumps, antibiotic modifying enzymes and limited membrane permeability. The primary reason for the development of novel therapeutics for P. aeruginosa infections is the declining efficacy of conventional antibiotic therapy. These clinical problems caused a revitalization of interest in bacteriophages, which are highly specific and have very effective antibacterial activity as well as several other advantages over traditional antimicrobial agents. Above all, so far, no serious or irreversible side effects of phage therapy have been described. Five newly purified P. aeruginosa phages named vB_PaeM_WP1, vB_PaeM_WP2, vB_PaeM_WP3, vB_PaeM_WP4 and vB_PaeP_WP5 have been characterized as potential candidates for use in phage therapy. They are representatives of the Myoviridae and Podoviridae families. Their host range, genome size, structural proteins and stability in various physical and chemical conditions were tested. The results of these preliminary investigations indicate that the newly isolated bacteriophages may be considered for use in phagotherapy.

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Acknowledgments

The electron microscopy studies were performed in the Laboratory of Electron Microscopy, University of Warmia and Mazury in Olsztyn, Poland (electron microscope JEM 1400, installed as part of a project sponsored by EU Structural Funds).

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Authors and Affiliations

  1. Biological Threat Identification and Countermeasure Centre, Military Institute of Hygiene and Epidemiology, Lubelska 2, 24-100, Puławy, Poland

    Magdalena Kwiatek, Lidia Mizak, Sylwia Parasion, Romuald Gryko & Marcin Niemcewicz

  2. Department of Medical Microbiology, Medical University of Lublin, Dr W. Chodzki 1, 20-093, Lublin, Poland

    Alina Olender

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  1. Magdalena Kwiatek
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  2. Lidia Mizak
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  3. Sylwia Parasion
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  4. Romuald Gryko
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  5. Alina Olender
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  6. Marcin Niemcewicz
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Correspondence to Magdalena Kwiatek.

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Kwiatek, M., Mizak, L., Parasion, S. et al. Characterization of five newly isolated bacteriophages active against Pseudomonas aeruginosa clinical strains. Folia Microbiol 60, 7–14 (2015). https://doi.org/10.1007/s12223-014-0333-3

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  • DOI: https://doi.org/10.1007/s12223-014-0333-3

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