Folia Microbiologica

, Volume 58, Issue 2, pp 155–162

Microbial control of the cotton leafworm Spodoptera littoralis (Boisd.) by Egyptian Bacillus thuringiensis isolates


  • Ahlam A. Alfazairy
    • Faculty of AgricultureAlexandria University
  • Amani M. D. El-Ahwany
    • Botany and Microbiology Department, Faculty of ScienceAlexandria University
  • Eman A. Mohamed
    • Faculty of ScienceDamanhour University
  • Heba A. H. Zaghloul
    • Botany and Microbiology Department, Faculty of ScienceAlexandria University
    • Botany and Microbiology Department, Faculty of ScienceAlexandria University
    • Faculty of Pharmacy and Drug ManufacturingPharos University in Alexandria

DOI: 10.1007/s12223-012-0193-7

Cite this article as:
Alfazairy, A.A., El-Ahwany, A.M.D., Mohamed, E.A. et al. Folia Microbiol (2013) 58: 155. doi:10.1007/s12223-012-0193-7


Four local Bacillus thuringiensis (Bt) isolates that had been serologically identified as Bt var. kurstaki (Btk2, Btk3, and Btk66) and Bt var. mexicanensis (Btm27), in addition to two reference strains (4D20 and 4AC1), were laboratory assayed as microbial control agents against the Egyptian cotton leafworm Spodoptera littoralis (Boisd.). Polymerase chain reaction (PCR) amplification analysis revealed that each of the six experimental strains carries, at least, a cry1 type gene which expresses a protein toxin active against lepidopterous insects. Additionally, PCR amplification results demonstrated that 4D20 and Btk66 contain the Lepidoptera- and Diptera-active cry2 type gene and that Btk66 contains Coleoptera-active cry7 and cry8 genes. Among the six strains, Btk66 and Btm27 were the most promising microbial control agents against S. littoralis. The present findings were the first to report that Btm27 (classified as B. thuringiensis var. mexicanensis) is a very potent microbial control agent against S. littoralis-tested larvae. For more characterization of these two isolates, the sspO gene was investigated as a molecular chronometer. The DNA sequencing results proved that Btk66 and Btm27 carry sspO open reading frames with identical nucleotide sequences, suggesting a strong phylogenetic relationship between the two strains.

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2012