Folia Microbiologica

, Volume 55, Issue 3, pp 277–280

Impact of heat-inactivated Lactobacillus casei and Lactobacillus paracasei strains on cytokine responses in whole blood cell cultures of children with atopic dermatitis

Authors

    • Department of PathologyThe Children’s Memorial Health Institute
  • I. Rosiak
    • Department of PathologyThe Children’s Memorial Health Institute
  • E. Klewicka
    • Institute of Fermentation Technology and MicrobiologyTechnical University of Lodz
  • I. Motyl
    • Institute of Fermentation Technology and MicrobiologyTechnical University of Lodz
  • M. Schwarzer
    • Institute of MicrobiologyAcademy of Sciences of the Czech Republic
  • Z. Libudzisz
    • Institute of Fermentation Technology and MicrobiologyTechnical University of Lodz
  • H. Kozakova
    • Institute of MicrobiologyAcademy of Sciences of the Czech Republic
Article

DOI: 10.1007/s12223-010-0041-6

Cite this article as:
Cukrowska, B., Rosiak, I., Klewicka, E. et al. Folia Microbiol (2010) 55: 277. doi:10.1007/s12223-010-0041-6

Abstract

Heat-inactivated Lactobacillus casei LOCK 0900, L. casei LOCK 0908 and Lactobacillus paracasei LOCK 0919 strains, applied to blood cell cultures obtained from children with atopic dermatitis induced production of anti-allergic TH1 cytokines (interleukin-12, interleukin-18, interferon-γ, tumor necrosis factor-α) and regulatory transforming growth factor-β1), but did not stimulate pro-allergic interleukin-5. The lactobacilli-mixture remarkably enhanced the TH1 response compared to single strains. This synergistic effect was not observed for transforming growth factor-β1. In contrast, the amount of interleukin-10 was found to be considerably lower when cells were stimulated with lactobacilli-mixture compared to single strains. The mixture of Lactobacillus strains represents a probiotic bacterial preparation modulating in vitro cytokine profile of allergic children towards anti-allergic TH1 response.

Abbreviations

IFN

interferon

IL

interleukin

PBS

phosphate-buffered saline

PHA

phytohemagglutinin

TGF

transforming growth factor

TH

T helper (lymphocytes)

TNF

tumor necrosis factor

Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2010