Article

Folia Microbiologica

, Volume 55, Issue 3, pp 277-280

Impact of heat-inactivated Lactobacillus casei and Lactobacillus paracasei strains on cytokine responses in whole blood cell cultures of children with atopic dermatitis

  • B. CukrowskaAffiliated withDepartment of Pathology, The Children’s Memorial Health Institute Email author 
  • , I. RosiakAffiliated withDepartment of Pathology, The Children’s Memorial Health Institute
  • , E. KlewickaAffiliated withInstitute of Fermentation Technology and Microbiology, Technical University of Lodz
  • , I. MotylAffiliated withInstitute of Fermentation Technology and Microbiology, Technical University of Lodz
  • , M. SchwarzerAffiliated withInstitute of Microbiology, Academy of Sciences of the Czech Republic
  • , Z. LibudziszAffiliated withInstitute of Fermentation Technology and Microbiology, Technical University of Lodz
  • , H. KozakovaAffiliated withInstitute of Microbiology, Academy of Sciences of the Czech Republic

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Abstract

Heat-inactivated Lactobacillus casei LOCK 0900, L. casei LOCK 0908 and Lactobacillus paracasei LOCK 0919 strains, applied to blood cell cultures obtained from children with atopic dermatitis induced production of anti-allergic TH1 cytokines (interleukin-12, interleukin-18, interferon-γ, tumor necrosis factor-α) and regulatory transforming growth factor-β1), but did not stimulate pro-allergic interleukin-5. The lactobacilli-mixture remarkably enhanced the TH1 response compared to single strains. This synergistic effect was not observed for transforming growth factor-β1. In contrast, the amount of interleukin-10 was found to be considerably lower when cells were stimulated with lactobacilli-mixture compared to single strains. The mixture of Lactobacillus strains represents a probiotic bacterial preparation modulating in vitro cytokine profile of allergic children towards anti-allergic TH1 response.