Abstract
The presence of phytase activity was demonstrated in 26 strains of rabbit cecal bacteria. In 25 strains a low phytase activity, 0.10–0.62 μmol phosphate released per min per mg protein, was found. High activity (2.61 μmol/min per mg protein) was found in the strain PP2 identified as Enterococcus hirae. Phytase activity was cell-associated, being higher in the cell extract than in the cell walls. Extracellular phytase activity and cell-associated phosphatase activity were not detected. Phytase activity was optimal around pH 5.0, which is below the physiological cecal pH range. The K m determined using the Lineweaver-Burk plot was 0.19 μmol/mL. Cations Fe3+, Cu2+ and Zn2+ at 0.5 mmol/L decreased phytase activity in sonicated cells of E. hirae by 99.4, 90.7 and 96.5 %, respectively. In contrast, Mg2+ increased activity by 11.0 %. Characteristics of E. hirae phytase (pH optimum, K m, cation sensitivity) were similar to those of other bacterial phytases reported in the literature. Other bacteria with a high phytase activity may be present in the rabbit cecum but remain to be identified.
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Marounek, M., Břeňová, N., Suchorská, O. et al. Phytase activity in rabbit cecal bacteria. Folia Microbiol 54, 111–114 (2009). https://doi.org/10.1007/s12223-009-0016-7
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DOI: https://doi.org/10.1007/s12223-009-0016-7