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Blend films based on silk fibroin/hyaluronic acid

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Abstract

Protein and polysaccharide was the most important extracellular matrix in dermal tissue. In this study, Silk fibroin (SF) / hyaluronic acid (HA) blend films mimicking the dermal tissue components were prepared and investigated. The results indicated that HA and SF has a good miscibility, HA interfered with SF to form crystal structure. By using EDC as cross-linker, effective cross-linking function on SF and HA macromolecules was reacted, the water solubility of the blend films decreased obviously after being cross-linked by EDC. The existence of EDC could promote SF to form Silk I structure. L929 cells were seeded on these blend films and showed normal attachment morphology. Cell-matrix interactions established by newly formed extracellular matrix were observed after 5 days in culture. The MTT assay showed that cell proliferation on the SF/HA blend films were enhanced significantly compared with that on the SF and HA films. These new 2D SF/HA blend films provided a favorable microenvironment for the proliferation of L929 cells and hold a potential for dermal tissue regeneration.

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Authors and Affiliations

  1. College of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073, China

    Shuqin Yan & Qiang Zhang

  2. College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, China

    Mingzhong Li, Qiang Zhang & Jiannan Wang

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  1. Shuqin Yan
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  2. Mingzhong Li
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  3. Qiang Zhang
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  4. Jiannan Wang
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Correspondence to Shuqin Yan.

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Yan, S., Li, M., Zhang, Q. et al. Blend films based on silk fibroin/hyaluronic acid. Fibers Polym 14, 188–194 (2013). https://doi.org/10.1007/s12221-013-0188-2

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  • DOI: https://doi.org/10.1007/s12221-013-0188-2

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