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Preparation and characterization of blended Bombyx mori silk fibroin scaffolds

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Abstract

The aim of this study was to compare physical, mechanical and biological properties of 3-dimensional scaffolds prepared from Bombyx mori silk fibroin (SF), fibroin blended with collagen (SF/C), and fibroin blended with gelatin (SF/G) using a freeze-drying technique. The prepared scaffolds were sponge-like structure that exhibited homogeneous porosity with highly interconnected pores. Average pore size of these scaffolds ranged from 65–147 µm. All biodegradable scaffolds were capable of water absorption of 90 %. The degradation behavior of these scaffolds could be controlled by varying the amount of blended polymer. The SF/C and SF/G scaffolds showed higher compressive modulus than that of SF scaffolds which could be attributed to the thicker pore wall observed in the blended constructs. The less crystalline SF structure was observed in SF/G scaffolds as compared to SF/C scaffolds. Thus, the highest compressive modulus was observed on SF/C matrix. To investigate the feasibility of the scaffolds for cartilage tissue engineering application, rat articular chondrocytes were seeded onto the scaffolds. The MTT assay demonstrated that blending collagen or gelatin into SF sponge facilitated cell attachment and proliferation better than SF scaffolds. The blended SF scaffolds possessed superior physical, mechanical and biological properties in comparison to SF scaffolds and showed high potential for application in cartilage tissue engineering.

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Authors and Affiliations

  1. Department of Pharmaceutical Technology and Center of Excellence for Innovation in Chemistry, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, 65000, Thailand

    Waree Tiyaboonchai & Pratthana Chomchalao

  2. Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok, 65000, Thailand

    Sutatip Pongcharoen

  3. Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand

    Manote Sutheerawattananonda

  4. Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Prasert Sobhon

Authors
  1. Waree Tiyaboonchai
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  2. Pratthana Chomchalao
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  3. Sutatip Pongcharoen
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  4. Manote Sutheerawattananonda
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  5. Prasert Sobhon
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Correspondence to Waree Tiyaboonchai.

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Tiyaboonchai, W., Chomchalao, P., Pongcharoen, S. et al. Preparation and characterization of blended Bombyx mori silk fibroin scaffolds. Fibers Polym 12, 324–333 (2011). https://doi.org/10.1007/s12221-011-0324-9

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  • DOI: https://doi.org/10.1007/s12221-011-0324-9

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