Abstract
Clinostats and centrifuges are widely used to create simulated microgravity or hypergravity, respectively, in order to study the impact of gravity on biosystems. Here, we used a clinostat and a centrifuge in alternating modes of operation in order to create a simulated parabolic flight like g-profile. To our knowledge, it is the first time that both devices were run in connection. In order to test the method, we investigated the production of reactive oxygen species of immune cells (macrophages) during oxidative burst in an on-line kinetic approach, which has been extensively studied under real (parabolic flight) and simulated microgravity (clinostat) as well as under hypergravity conditions (centrifuge). Our results indicate that clinostat and centrifuge can be operated in an alternating way to simulate the repetitive changes of gravity during parabolic flight. Although the switch from one gravity level to the other could not be carried out as quickly as it takes place during actual parabolic flight due to technical and operational reasons, it can be concluded that running experiments in a clinostat aboard a centrifuge on ground are suitable for studying gravity-related phenomena.
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References
Abbas, A., Lichtman, A., Pillai, S.: Cellular and Molecular Immunology, 6th edn. Saunders/Elsevier, Philadelphia (2010)
Adrian, A., Schoppmann, K., Sromicki, J., Brungs, S., von der Wiesche, M., Hock, B., Kolanus, W., Hemmersbach, R., Ullrich, O.: The oxidative burst reaction in mammalian cells depends on gravity. J. Cell Commun. Signal 11, 98 (2013)
Brungs, S.: The influence of altered gravity on the oxidative burst in macrophages. Dissertation, University of Bonn (2013)
Brungs, S., Kolanus, W., Hemmersbach, R.: Impact of altered gravity on the oxidative burst in macrophages. J. Cell Commun. Signal 13, 9 (2015)
Frett, T., Mayrhofer, M., Schwandtner, J., Anken, R., Petrat, G.: An innovative short arm centrifuge for future studies on the effects of artificial gravity on the human body. Microgravity Sci. Technol. 26, 249 (2014)
Horn, A., Ulrich, O., Huber, K., Hemmersbach, R.: PMT (Photomultiplier) Clinostat. MST 23(1) (2010)
Horn, A.: Vorbereitungen für das Biolab Experiment TRIPLE LUX A Hardwareentwicklung, Kalibrierung und biologische Bodenkontrollen. Dissertation, Otto-von-Guericke-University, Magdeburg (2011)
Huber, K.: Phagozytose und oxidative Burst als Biomarker für Immuntoxizität – Der Einfluss von simulierter Schwerelosigkeit auf die Makrophagenzelllinie NR8383. Dissertation, Technical University of Munich (2009)
Zander, V., Anken, R., Pesquet, T., Brungs, S., Latsch, J.: Short radius centrifuges – A new approach for life science experiments under hyper-g conditions for applications in space and beyond. Recent Progr. Space Technol. 3, 74 (2013)
Acknowledgments
The authors gratefully thank ESA (GBF Project CORA-GBF-400012843).
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The authors declare that they have no conflict of interest.
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Brungs, S., Petrat, G., der Wiesche, M.v. et al. Simulating Parabolic Flight like g-Profiles on Ground - A Combination of Centrifuge and Clinostat. Microgravity Sci. Technol. 28, 231–235 (2016). https://doi.org/10.1007/s12217-015-9458-5
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DOI: https://doi.org/10.1007/s12217-015-9458-5