Abstract
Seedlings of Arabidopsis thaliana-ABD2-GFP were grown under slow clinorotation (2 rpm) and treated with actin and tubulin disrupting drugs in order to characterize the role of actin microfilaments in cell growth and gravisensing. Changes in microfilament organization and cell parameters have shown that the transition root zone (TZ) is rather sensitive to microfilament disruption in control plants. It is assumed that under clinorotation, organization of actin cytoskeleton in the TZ is coordinated in a different way than in the control. Organization of microfilaments depends upon organization of microtubules and clinorotation does not influence this interrelation significantly.
Similar content being viewed by others
References
Baluška, F., Volkmann, D., Barlow, P.: A polarity crossroad in the transition growth zone of maize root apices: cytoskeletal and developmental implications. J. Plant Growth Regul. 20, 170–181 (2001)
Baluška, F., Mancuso, S., Volkmann, D., Barlow, P.W.: Root apex transition zone:a signalling—response nexus in the root. Trends Plant Sci. 15(7), 402–408 (2010)
Blancaflor, E.B.: Cortical actin filaments potentially interact with cortical microtubules in regulating polarity of cell expansion in primary roots of maize (Zea mays L.). J. Plant Growth Regul. 19, 406–414 (2000)
Blancaflor, E.B.: The cytoskeleton and gravitropism in higher plants. J. Plant Growth Regul. 21, 120–136 (2002)
Collings, D.A.: Crossed-wires: interactions and cross-talk between the microtubule and microfilament networks in plants. In: Nick, P. (ed.) Plant Microtubules: Development and Flexibility, pp. 47–79. Springer, Berlin (2008)
Collings, D., Lill, A., Himmelspach, R., Wasteneys, G.: Hypersensitivity to cytoskeletal antagonists demonstrates microtubule-microfilament cross-talk in the control of root elongation in Arabidopsis thaliana. New Phytol. 170, 275–290 (2006)
Fischer, K., Schopfer, P.: Physical strain-mediated microtubule reorientation in the epidermis of gravitropically or phototropically stimulated maize coleoptiles. Plant J. 15, 119–123 (1998)
Fu, Y., Gu, Y., Zheung, Z., Wasteneys, G., Yang, Z.: Arabidopsis interdigitating cell growth requires two antagonistic pathways with opposing action on cell morphogenesis. Cell 120, 687–700 (2005)
Lang, T., Wacker, I., Wunderlich, I., Rohrbach, A., Giese, G., et al.: Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells. Biophys. J. 78, 2863–2877 (2000)
Li, Y., Shen, Y., Cai, C., Zhong, C., Zhu, L., Yuan, M., Rena, H.: The type II Arabidopsis formin14 interacts with microtubules and microfilaments to regulate cell division. Plant Cell 22, 2710–2726 (2010)
Lloyd, C.W., Chan, J.: Microtubules and the shape of plants to come. Nat. Rev. Mol. Cell Biol. 5, 13–23 (2004)
Mancuso, S., Boselli, M.: Characterization of the oxygen fluxes in the division, elongation and mature zones of Vitis roots: influence of oxygen availability. Planta 214, 767–774 (2002)
Mancuso, S., Marras, A.M., Magnus, V., Baluška, F.: Noninvasive and continuous recordings of auxin fluxes in intact root apex with a carbon nanotube-modified and self-referencing microelectrode. Anal. Biochem. 341, 344–351 (2005)
Papaseit, C., Pochon, N., Tabony, J.: Microtubule self-organization is gravity-dependent. Proc. Natl. Acad. Sci. 97, 8364–8368 (2000)
Petraśek, J., Schwarzerova, K.: Actin and microtubule cytoskeleton interactions. Curr. Opin. Plant Biol. 12, 728–734 (2009)
Sampathkumar, A., Lindeboom, J., Debolt, S., Gutierrez, R., Ehrhardt, D., Ketelaar, T., Perssona, S.: Live cell imaging reveals structural associations between the actin and microtubule cytoskeleton in Arabidopsis. Plant Cell 23, 2302–2313 (2011)
Shevchenko, G.: Patterns of cortical microtubules formed in epidermis of Beta vulgaris roots under clinorotation. Adv. Space Res. 24, 739–742 (1999)
Shevchenko, G., Kalinina, Ia., Kordyum, E.L.: Interrelation between microtubules and microfilaments in the elongation zone of Arabidopsis root under clinorotation. Adv. Space Res. 39, 1171–1175 (2007)
Smith, L.G., Oppenheimer, D.G.: Spatial control of cell expansion by the plant cytoskeleton. Annu. Rev. Cell Dev. Biol. 21, 271–295 (2005)
Wang, Y.S., Yoo, C.M., Blancaflor, E.B.: Improved imaging of actin filaments in transgenic Arabidopsis plants expressing a green fluorescent protein fusion to the C- and N-termini of the fimbrin actin-binding domain. New Phytol. 177, 525–36 (2008)
Wasteneys, G., Galway, M.: Remodeling the cytoskeleton for growth and form: an overview with some new views. Annu. Rev. Plant Biol. 54, 691–722 (2003)
Wasteneys, G.O., Yang, Z.: New views on the plant cytoskeleton. Plant Physiol. 136, 3884–3891 (2004)
Wiesler, B., Wang, Q.Y., Nick, P.: The stability of cortical microtubules depends on their orientation. Plant J. 32, 1023–1032 (2002)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shevchenko, G. Actin Microfilament Organization in the Transition Zone of Arabidopsis-ABD2-GFP Roots Under Clinorotation. Microgravity Sci. Technol. 24, 427–433 (2012). https://doi.org/10.1007/s12217-012-9318-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12217-012-9318-5