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Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity Conditions

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Abstract

In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a rotating wall vessel to simulate microgravity (SMG) and in a control setup. The differences in Raman spectra recorded from both setups after 24 h of culturing were small. The most prominent features in a difference spectrum, calculated between the mean spectra from the microgravity and the control setup separately, could be assigned to the presence of poly-β-hydroxybutyrate (PHB). SMG seems to yield a higher PHB production after 24 h of culturing. Additional processing of the spectra suggested that SMG induced also other changes in the carbon-metabolism. After 48 h, similar results were found for the carbon metabolism, while PHB concentrations were reduced in SMG compared to the control. However, these differences could also be caused by interfering effects that may occur in the bioreactors after a prolonged incubation time.

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Author notes

  1. Patrick De Boever

    Present address: Environmental Toxicology, Flemish Institute for Technological Research, Retieseweg, 2240, Geel, Belgium

Authors and Affiliations

  1. Department of Analytical Chemistry, Ghent University, Proeftuinstraat 86, 9000, Ghent, Belgium

    Joke De Gelder & Luc Moens

  2. Department of Archeology and Ancient History of Europe, Ghent University, Blandijnberg 2, 9000, Ghent, Belgium

    Peter Vandenabeele

  3. Laboratory for Molecular and Cellular Biology, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK•CEN), Boeretang 200, 2400, Mol, Belgium

    Patrick De Boever & Max Mergeay

  4. Laboratory of Microbiology (Lm-Ugent), Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium

    Paul De Vos

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  1. Joke De Gelder
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  2. Peter Vandenabeele
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Correspondence to Joke De Gelder.

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De Gelder, J., Vandenabeele, P., De Boever, P. et al. Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity Conditions. Microgravity Sci. Technol 21, 217–223 (2009). https://doi.org/10.1007/s12217-008-9037-0

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  • DOI: https://doi.org/10.1007/s12217-008-9037-0

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