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Micropropagation of Clerodendrum L. species: a review

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Abstract

Species within the Clerodendrum genus possess well-established pharmacological and medicinal properties. These emanate from the presence of many unique bioactive secondary metabolites. Eight Clerodendrum species (C. anomalum, C. calcicola, C. denticulatum, C. galeatum, C. leucophloeum, C. lutambense, C. eupatorioides, and C. aculeatum) are listed on the International Union of Conservation of Nature Red Data List. Micropropagation offers an opportunity for the conservation and large-scale propagation of elite genotypes and is a widely used technique for the conservation of threatened medicinal plants, including Clerodendrum species. This review highlights the advances that have been made thus far in the in vitro tissue culture of members of the Clerodendrum genus. Most of the efforts made to date have focussed on basic in vitro growth or regeneration, primarily from seeds, nodes, and leaves, either from an ex vitro or an in vitro source. Following a wide range of disinfection procedures that depend on the source and age of the explant, most studies employed Murashige and Skoog basal medium in a 12- to 16-h photoperiod. The most effective cytokinin for the induction of shoots was N 6-benzyladenine. Although plantlet acclimatization to field conditions was widely reported, it was often poorly quantified. Based on this base of information, applied aspects such as genetic engineering, mutation breeding, the production of haploid and double-haploid lines, protoplast fusion, embryo rescue, in vitro production of secondary metabolite acquisition through hairy roots or bioreactors, somaclonal variation, and cryopreservation still need to be developed and explored.

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References

  • Ashajyothi V, Fatima J (2013) Review on Clerodendrum phlomidis (Agnimantha). Int J Pharm Res Rev 1:423–428

    Google Scholar 

  • Ashmore SE, Hamilton KN, Offord CA (2011) Conservation technologies for safeguarding and restoring threatened flora: case studies from Eastern Australia. In Vitro Cell Dev Biol Plant 47:99–109

    Article  Google Scholar 

  • Baburaj S, Ravichandran P, Selvapandi M (2000) In vitro adventitious shoot formation from leaf cultures of Clerodendrum inerme. Indian J Exp Biol 38:1274–1276

    CAS  Google Scholar 

  • Bairu MW, Aremu AO, Van Staden J (2010) Somaclonal variation in plants: causes and detection methods. Plant Growth Regul 63:147–173

    Article  Google Scholar 

  • Cardoso JC, Rossi ML, Rosalem IB, Teixeira da Silva JA (2013) Pre-acclimatization in the greenhouse: an alternative to optimizing the micropropagation of Gerbera. Sci Hortic 164:616–624

    Article  CAS  Google Scholar 

  • Chandra S (2012) Natural plant genetic engineer Agrobacterium rhizogenes: role of T-DNA in plant secondary metabolism. Biotechnol Lett 34:407–415

    Article  CAS  Google Scholar 

  • Chattopadhyay P, Chatterjee S, Sen S (2008) Biotechnological potential of natural food grade biocolorants. Afr J Biotechnol 7:2972–2985

    CAS  Google Scholar 

  • Devika R, Kovilpillai J (2012) Phytochemical and in vitro micropropagation studies of Clerodendrum phlomidis L. J Pharm Res 5:4396–4398

    CAS  Google Scholar 

  • Engelmann F (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell Dev Biol Plant 47:5–16

    Article  Google Scholar 

  • Farooq S, Farook T, Al-Rawahy S (2010) High-frequency micropropagation and somatic embryogenesis from axillary bud and leaf explants of Clerodendrum inerme. In Vitro Cell Dev Biol Animal 46:113–114

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50(1):151–158

    Article  CAS  Google Scholar 

  • George EF, Hall MA, De Klerk G-J (2008a) The components of plant tissue culture media I: macro- and micro-nutrient. In: George EF, Hall MA, De Klerk G-J (eds) Plant propagation by tissue culture. Springer, Dordrecht, the Netherlands, pp 65–113

  • George EF, Hall MA, De Klerk G-J (2008b) The components of plant Tissue culture media ll: organic additions, osmotic and pH effects, and support systems. In: George EF, Hall MA, De Klerk G-J (eds) Plant propagation by tissue culture. Springer, Dordrecht, the Netherlands, pp 115–173

  • Goyal S, Shahzad A, Anis M, Khan S (2010) Multiple shoot regeneration in Clerodendrum incisum L.—an ornamental woody shrub. Pak J Bot 42:873–878

    CAS  Google Scholar 

  • International Union for Conservation of Nature and Natural Resources (IUCN) (2015). The IUCN Red List of Threatened Species ver 2015.2. http://www.iucnredlist.org/ Accessed 10 Oct 2015

  • Jirakiattikul Y, Boonha K (2009) In vitro proliferation and root induction of Clerodendrum wallichii. Thai Sci Technol J 17(3):61–67 and 17(4):101–102

  • Kamdi SR, Neharkar PS, Kadu PR, Bhagat GJ, Pathan IA, Roy NS (2014) Micro propagation and optimization of protocol for medicinal important plant (Clerodendrum viscosum). Int J Plant Sci 9:389–393

    Google Scholar 

  • Kothari A, Padh H, Shrivastava N (2006) Ex situ conservation method for Clerodendrum inerme: a medicinal plant of India. African J Biotechnol 5:415–418

    Google Scholar 

  • Krishnan PN, Decruse SW, Radha RK (2011) Conservation of medicinal plants of Western Ghats, India and its sustainable utilization through in vitro technology. In Vitro Cell Dev Biol Plant 47:110–122

    Article  Google Scholar 

  • Krutovsky KV, Tretyakova IN, Oreshkova NV, Pak ME, Kvitko OV, Vaganov EA (2014) Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch (Larix sibirica Ledeb.) megagametophytes for whole genome de novo sequencing. In Vitro Cell Dev Biol Plant 50:655–664

    Article  CAS  Google Scholar 

  • Kulus D, Zalewska M (2014) Cryopreservation as a tool used in long-term storage of ornamental species—a review. Sci Hortic 168:88–107

    Article  Google Scholar 

  • Lema-Rumińska J, Kulus D (2014) Micropropagation of cacti—a review. Haseltonia 19:46–63

    Article  Google Scholar 

  • Lloyd G, McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Int Plant Propagators’ Soc Proc 30:421–427

    Google Scholar 

  • Ma JK-C, Chikwamba R, Sparrow P, Fischer R, Mahoney R, Twyman RM (2005) Plant-derived pharmaceuticals—the road forward. Trends Plant Sci 10:580–585

    Article  CAS  Google Scholar 

  • Mao AA, Wetten A, Fay M, Caligari PD (1995) In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant. Plant Cell Rep 14:493–496

    Article  CAS  Google Scholar 

  • Miguel C, Marum L (2011) An epigenetic view of plant cells cultured in vitro: somaclonal variation and beyond. J Exp Bot 62:3713–3725

    Article  CAS  Google Scholar 

  • Mukherjee A, Bandyopadhyay A (2014) Inducing somatic embryogenesis by polyamines in medicinally important Clerodendrum indicum L. Int J Curr Microbiol Appl Sci 3:12–26

    CAS  Google Scholar 

  • Mukherjee A, Dutta S, Bandyopadhyay A (2012) Micropropagation of Clerodendrum indicum (L.) Kuntze: an unexplored medicinal plant. Int J Pharma Bio Sci 3:659–668

    CAS  Google Scholar 

  • Mukherjee A, Bandyopadhyay A, Dutta S, Basu S (2013) Phytoaccumulation of iron by callus tissue of Clerodendrum indicum (L.). Chem Ecol 29:564–571

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Norikane A, Teixeira da Silva JA, Tanaka M (2013) Growth of in vitro Oncidesa plantlets cultured under cold cathode fluorescent lamps with super-elevated CO2 enrichment. AoB Plants plt044

  • Oksman-Caldentey K-M, Inzé D (2004) Plant cell factories in the post-genomic era: new ways to produce designer secondary metabolites. Trends Plant Sci 9:433–440

    Article  CAS  Google Scholar 

  • Parmar VR, Patel HA, Jasrai YT (2013) Developing normal plants of Clerodendron from viral infected stock through meristem culture. Cibtech J Bio-Protocols 2:1–5

    CAS  Google Scholar 

  • Pospíšilová J, Tichá I, Kadlechek P, Haisel D, Plzakova S (1999) Acclimatization of micropropagated plants to ex vitro conditions. Biol Plant 42:481–497

    Article  Google Scholar 

  • Prakash R (2013) Medicinal value of Clerodendrum phlomidis: a review. Int J Pharmacol 7:1–7

    Google Scholar 

  • Raaman N, Divakar S, Jeyam P, Hariprasath L, Baskar M, Mathiyazhagan K (2011) Micropropagation, antimicrobial activity and phytochemical analysis of Clerodendron phlomidis Linn. Med Plants 3:119–127

    Google Scholar 

  • Raja MKMM, Mishra SH (2010) Comprehensive review of Clerodendrum phlomidis: a traditionally used bitter. J Chin Integr Med 8:510–524

    Article  CAS  Google Scholar 

  • Sharma M, Rai SK, Purshottam DK, Jain M, Chakrabarty D, Awasthi A, Nair KN, Sharma AK (2009) In vitro clonal propagation of Clerodendrum serratum (Linn.) Moon (barangi): a rare and threatened medicinal plant. Acta Physiol Plant 31:379–383

    Article  Google Scholar 

  • Sharma S, Shahzad A, Teixeira da Silva JA (2013) Synseed technology—a complete synthesis. Biotechnol Adv 31:186–207

    Article  CAS  Google Scholar 

  • Shrivastava N, Patel T (2007a) Clerodendrum and healthcare: an overview. Med Aromat Plant Sci Biotechnol 1:142–150

    Google Scholar 

  • Shrivastava N, Patel T (2007b) Clerodendrum and healthcare: an overview—part II phytochemistry and biotechnology. Med Aromat Plant Sci Biotechnol 1:209–223

    Google Scholar 

  • Srinath G, Sridhar V, Renuka R (2009) In vitro propagation of the medicinal herb—Clerodendrum inerme. Adv Biotechnol 9:21–23

    Google Scholar 

  • Srivastava A, Gupta RK, Verma HN (2004) Micropropagation of Clerodendrum aculeatum through adventitious shoot induction and production of consistent amount of virus resistance inducing protein. Indian J Exp Biol 42:1200–1207

    CAS  Google Scholar 

  • Teixeira da Silva JA (2012a) Is BA (6-benzyladenine) BAP (6-benzylaminopurine)? Asian Aust J Plant Sci Biotechnol 6 (special issue 1): 121–124

  • Teixeira da Silva JA (2012b) Callus, calluses or calli: multiple plurals? Asian Aust J Plant Sci Biotechnol 6 (special issue 1): 125–126

  • Teixeira da Silva JA (2014) The response of protocorm-like bodies of nine hybrid Cymbidium cultivars to light-emitting diodes. Environ Exp Biol 12:155–159

    Google Scholar 

  • Teixeira da Silva JA, Dobránszki J (2013) Plant thin cell layers: a 40-year celebration. J Plant Growth Reg 32(4):922–943

    Article  CAS  Google Scholar 

  • Teixeira da Silva JA, Giang DDT, Tanaka M (2005) In vitro acclimatization of banana and Cymbidium. Int J Bot 1:41–49

    Article  Google Scholar 

  • Teixeira da Silva JA, Bolibok H, Rakoczy-Trojanowska M (2007) Molecular markers in micropropagation, tissue culture and in vitro plant research. Genes Genomes Genomics 1:66–72

    Google Scholar 

  • Teixeira da Silva JA, Zeng S, Cardoso JC, Dobránszki J, Kerbauy GB (2014) In vitro flowering of Dendrobium. Plant Cell, Tissue Organ Cult 119:447–456

    Article  CAS  Google Scholar 

  • Teixeira da Silva JA, Winarto B, Dobránszki J, Zeng S (2015) Anther culture of Anthurium: a review. Acta Physiol Plantarum 37:173. doi:10.1007/s11738-015-1909-5

    Article  Google Scholar 

  • The Plant List (2015). http://www.theplantlist.org/tpl1.1/search?q=Clerodendrum. Accessed 10 Oct 2015

  • Thimijan RW, Heins RD (1983) Photometric, radiometric, and quantum light units of measure: a review of procedures for interconversion. HortScience 18:818–822

    Google Scholar 

  • Vidya SM, Krishna V, Manjunatha BK (2005) Micropropagation of Clerodendrum serratum L. from leaf explants. J Non-Timber For Prod 12:57–60

    Google Scholar 

  • Vidya SM, Krishna V, Manjunatha BK, Pradeepa (2012) Micropropagation of Clerodendrum serratum L. through direct and indirect organogenesis. Plant Tissue Cult Biotechnol 22:179–185

    Google Scholar 

  • Wang Q-M, Wang L (2012) An evolutionary view of plant tissue culture: somaclonal variation and selection. Plant Cell Rep 31:1535–1547

    Article  CAS  Google Scholar 

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Acknowledgments

The authors thank Dr. Yaowapha Jirakiattikul, Department of Agricultural Technology, Faculty of Science and Technology, Thammasat University (Thailand), for providing detailed information about the Jirakiattikul and Boonha (2009) reference. We are also thankful to Professor N. Raaman (Herbal Sciences Laboratory, Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, India), and Dr. S. M. Vidya (Department of Biotechnology, NMAM Institute of Technology, Karnataka, India) for literature support. Thanks are also extended to the anonymous reviewers for their comments that allowed for the improvement of this manuscript.

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  1. B.R. Doshi School of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Rd., P.O. Box 39, Vallabh Vidyanagar, Gujarat, 388120, India

    M. Nataraj & Mafatlal M. Kher

  2. P. O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, Miki, Kagawa-ken, 761-0799, Japan

    Jaime A. Teixeira da Silva

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  1. M. Nataraj
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Correspondence to M. Nataraj, Mafatlal M. Kher or Jaime A. Teixeira da Silva.

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Nataraj, M., Kher, M.M. & Teixeira da Silva, J.A. Micropropagation of Clerodendrum L. species: a review. Rend. Fis. Acc. Lincei 27, 169–179 (2016). https://doi.org/10.1007/s12210-015-0484-4

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