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In vivo imaging of a single erythrocyte with high-resolution photoacoustic microscopy

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Abstract

In this letter, we reported a high-resolution photoacoustic microscopy (PAM) to image erythrocytes and blood vessels. The developed system had the ability to provide a lateral resolution of 1.0 μm at the wavelength of 532 nm with a × 10 objective. First, we used a sharp edge to measure the lateral resolution of the PAM and testified the stability with carbon fibers. Then, using this system, in vivo blood vessels and capillaries of a mouse ear, even a single erythrocyte can be clearly imaged. There was a pair of accompanying venule and arteriole, whose detailed and further complicated branches can be clearly identified. And likely red blood cells (RBCs) arrayed one by one in microvasculature was also shown. The experimental results demonstrate that the high-resolution PAM has potential clinical applications for imaging of erythrocytes and blood vessels.

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Author information

Authors and Affiliations

  1. Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science College of Biophotonics, South China Normal University, Guangzhou, 510631, China

    Guo He, Bingbing Li & Sihua Yang

Authors
  1. Guo He
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  2. Bingbing Li
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  3. Sihua Yang
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Corresponding author

Correspondence to Sihua Yang.

Additional information

Guo He received her B.S. degree, major in Optical Information Science and Technology, from Tianjin Polytechnic University in 2012. Then she joined Ministry of Education (MOE) Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University for her master degree. Her present work is focused on photoacoustic flow cytography and its related biomedical applications.

Bingbing Li earned her bachelor degree from Huaibei Normal University in 2011, and her master degree in Prof. Da Xing’s group at Ministry of Education (MOE) Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University in 2014. She enjoyed challenging areas of studies such as fast variable focus photoacoustic microscopy and its practical characteristics, and the research article has been published on international journals.

Sihua Yang received his doctoral degree in Optics in 2009 at South China Normal University. Now he is Professor, the Vice Dean, College of Biophotonics, Institute of Life Science, and South China Normal University. He got the “Sylvia Sorkin Greenfield Award” of American Association of Physicists in Medicine (AAPM) in 2008, and the Natural Science Award of Guangdong Province in 2008 and 2013. His main interests in research include photoacoustic molecular imaging and clinical applications, multi-modality imaging of photoacoustics, ultrasound and fluorescence, ultrashort microwave-induced thermoacoustic imaging.

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He, G., Li, B. & Yang, S. In vivo imaging of a single erythrocyte with high-resolution photoacoustic microscopy. Front. Optoelectron. 8, 122–127 (2015). https://doi.org/10.1007/s12200-014-0461-z

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  • DOI: https://doi.org/10.1007/s12200-014-0461-z

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