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Influence of A-21T and C-262T genetic polymorphisms at the promoter region of the catalase (CAT) on gene expression

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Environmental Health and Preventive Medicine Aims and scope

Abstract

Objective

Catalase (CAT, OMIM: 115500) is one of the major antioxidant enzymes, which plays an important role in the clearance of reactive oxygen species. Three genetic polymorphisms of A-21T (rs7943316), C-262T (rs1001179), and C-844T (rs769214) in the promoter region of the CAT have been reported. It has been suggested that these polymorphisms may alter the recognition sites of transcriptional factors, therefore it might be concluded that these polymorphisms may alter the expression levels of the gene. The aim of the present study is to evaluate the associations between these genetic variations and the CAT mRNA levels in human peripheral blood cells.

Methods

The present study consisted of 47 healthy students of Shiraz University (south-west Iran). Genotypes of the CAT polymorphisms were determined by PCR based method. The quantitative CAT mRNA expression levels were investigated using quantitative real-time PCR.

Results

Analysis of variance revealed significant differences between the study genotypes (For A-21T polymorphism: F = 7.45; df = 2, 44; P = 0.002; For C-262T polymorphism: F = 15.17; df = 2, 44; P < 0.001). The studied polymorphisms showed linkage disequilibrium (D′ = 1.0, r 2 = 0.1813, χ 2 = 17.03, P < 0.0001). The mRNA levels of CAT in the AC/TT, TC/TC, TC/TT, and TC/TC diplotypes significantly were higher than the mRNA levels in AC/AC diplotype. There was a significant difference between the study genotypes (F = 9.24; df = 5, 41; P < 0.001). The TC/TC and TT/TT diplotypes showed about 2 and 4 folds CAT mRNA levels compared with the AC/AC diplotype.

Conclusions

The present findings indicated that these polymorphisms were significantly associated with the gene expression.

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References

  1. Ogata M. Acatalasemia. Hum Genet. 1991;86:331–40.

    Article  CAS  PubMed  Google Scholar 

  2. Forsberg L, Lyrenäs L, de Faire U, Morgenstern R. A common functional C-T substitution polymorphism in the promoter region of the human catalase gene influences transcription factor binding, reporter gene transcription and is correlated to blood catalase levels. Free Radic Biol Med. 2001;30:500–5.

    Article  CAS  PubMed  Google Scholar 

  3. Lourdhu Mary A, Nithya K, Isabel W, Angeline T. Prevalence of catalase (-21A/T) gene variant in south Indian (Tamil) population. Biomed Res Int. 2014;2014:894237.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Kodydková J, Vávrová L, Kocík M, Žák A. Human catalase, its polymorphisms, regulation and changes of its activity in different diseases. Folia Biol (Praha). 2014;60:153–67.

    Google Scholar 

  5. Saadat M, Saadat S. Genetic polymorphism of CAT C-262 T and susceptibility to breast cancer, a case-control study and meta-analysis of the literatures. Pathol Oncol Res. 2015;21:433–7.

    Article  CAS  PubMed  Google Scholar 

  6. Ebrahimpour S, Saadat I. Association of CAT C-262T and SOD1 A251G single nucleotide polymorphisms susceptible to gastric cancer. Mol Biol Res Commun. 2015;4:223–9.

    Google Scholar 

  7. Zarei N, Saadat I, Farvardin-Jahromi M. The relationship between NQO1 C609T and CAT C-262T genetic polymorphisms and the risk of age-related cataracts. Mol Biol Res Commun. 2015;4:143–9.

    PubMed  PubMed Central  Google Scholar 

  8. Hu J, Feng F, Zhu S, Sun L, Li G, Jiang N, Shang Z, Niu Y. Catalase C-262T polymorphism and risk of prostate cancer: evidence from meta-analysis. Gene. 2015;558:265–70.

    Article  CAS  PubMed  Google Scholar 

  9. Shen Y, Li D, Tian P, Shen K, Zhu J, Feng M, Wan C, Yang T, Chen L, Wen F. The catalase C-262T gene polymorphism and cancer risk: a systematic review and meta-analysis. Med (Baltim). 2015;94:e679.

    Article  CAS  Google Scholar 

  10. Saygi S, Erol İ, Alehan F, Yalçın YY, Kubat G, Ataç FB. Superoxide dismutase and catalase genotypes in pediatric migraine patients. J Child Neurol. 2015;30:1586–90.

    Article  PubMed  Google Scholar 

  11. Góth L, Vitai M. Polymorphism of 5′ of the catalase gene in Hungarian acatalasemia and hypocatalasemia. Electrophoresis. 1997;18:1105–8.

    Article  PubMed  Google Scholar 

  12. Wang SL, Yu LG, Liu RP, Zhu WZ, Gao WM, Xue LP, Jiang X, Zhang YH, Yi D, Chen D, Zhang YH. Gene–gene interaction of GJB2, SOD2, and CAT on occupational noise-induced hearing loss in Chinese Han population. Biomed Environ Sci. 2014;27:965–8.

    CAS  PubMed  Google Scholar 

  13. Vialykh EK, Solidolova MA, Bushueva O, Bulgakova IV, Polonikov AV. Catalase gene polymorphism is associated with increased risk of cerebral stroke in hypertensive patients. Zh Nevrol Psikhiatr Im S S Korsakova. 2012;112:3–7 (in Russian).

    CAS  PubMed  Google Scholar 

  14. Saify K. Genetic polymorphisms in the promoter region of catalase gene, creates a new potential PAX-6 and STAT4 response element. Mol Biol Res Commun. 2016;5:97–100.

    PubMed  PubMed Central  Google Scholar 

  15. Hebert-Schuster M, Cottart CH, Laguillier-Morizot C, Raynaud-Simon A, Golmard JL, Cynober L, Beaudeux JL, Fabre EE, Nivet-Antoine V. Catalase rs769214 SNP in elderly malnutrition and during renutrition: is glucagon to blame? Free Radic Biol Med. 2011;51:1583–8.

    Article  CAS  PubMed  Google Scholar 

  16. Saify K, Saadat M. Expression patterns of antioxidant genes in human SH-SY5Y cells after treatment with methadone. Psychiatry Res. 2015;230:116–9.

    Article  CAS  PubMed  Google Scholar 

  17. Saify K, Saadat I, Saadat M. Down-regulation of antioxidant genes in human SH-SY5Y cells after treatment with morphine. Life Sci. 2015;144:26–9.

    Article  PubMed  Google Scholar 

  18. Rafiee L, Saadat I, Saadat M. Glutathione S-transferase genetic polymorphisms (GSTM1, GSTT1 and GSTO2) in three Iranian populations. Mol Biol Rep. 2010;37:155–8.

    Article  CAS  PubMed  Google Scholar 

  19. Saadat M. Distribution of ACE insertion/deletion (I/D) polymorphism in Iranian populations. Mol Biol Res Commun. 2015;4:63–6.

    PubMed  PubMed Central  Google Scholar 

  20. Saadat M, Mousavi-Kazerooni, Fallahzadeh-Abarghooei L. Distribution of genetic polymorphism of CAT C-262T in three Iranian populations. Turk J Biochem. 2015;40:386–9.

    CAS  Google Scholar 

  21. Fallahzadeh-Abarghooei L, Zahedi T, Mirabedi F, Saadat M. Alleleic prevalence of intron 3 insertion/deletion genetic polymorphism of DNA double-strand break repair gene XRCC4 in four Iranian populations. Egypt J Med Hum Genet. 2015;16:215–8.

    Article  Google Scholar 

  22. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc. 2008;3:1101–8.

    Article  CAS  PubMed  Google Scholar 

  23. Ahn J, Nowell S, McCann SE, Yu J, Carter L, Lang NP, Kadlubar FF, Ratnasinghe LD, Ambrosone CB. Associations between catalase phenotype and genotype: modification by epidemiologic factors. Cancer Epidemiol Biomark Prev. 2006;15:1217–22.

    Article  CAS  Google Scholar 

  24. Nadif R, Mintz M, Jedlicka A, Bertrand JP, Kleeberger SR, Kauffmann F. Association of CAT polymorphisms with catalase activity and exposure to environmental oxidative stimuli. Free Radic Res. 2005;39(12):1345–50.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Mak JCW, Ho SP, Yu WC, Choo KL, Chu CM, Yew WW, Lam WK, Chan-Yeung MW. Polymorphisms and functional activity in superoxide dismutase and catalase genes in smokers with COPD. Eur Respir J. 2007;30:684–90.

    Article  CAS  PubMed  Google Scholar 

  26. Smaga I, Niedzielska E, Gawlik M, Moniczewski A, Krzek J, Przegaliński E, Pera J, Filip M. Oxidative stress as an etiological factor and a potential treatment target of psychiatric disorders. Part 2. Depression, anxiety, schizophrenia and autism. Pharmacol Rep. 2015;67:569–80.

    Article  CAS  PubMed  Google Scholar 

  27. Beebe DC, Holekamp NM, Shui YB. Oxidative damage and the prevention of age-related cataracts. Ophthalmic Res. 2010;44:155–65.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. He F, Zuo L. Redox roles of reactive oxygen species in cardiovascular diseases. Int J Mol Sci. 2015;16:27770–80.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Mahmoudinasab H, Sanie-Jahromi F, Saadat M. Effects of extremely low-frequency electromagnetic field on expression levels of some antioxidant genes in MCF-7 cells. Mol Biol Res Commun. 2016;5:77–85.

    PubMed  PubMed Central  Google Scholar 

  30. Badr AA, Saadat I, Saadat M. Study of liver function and expression of some detoxification genes in the male rats exposed to methyl-tertiary butyl ether. Egypt J Med Hum Genet. 2016. doi: 10.1016/j.2ejmhg.2015.10.002.

  31. Higdon JV, Delage B, Williams DE, Dashwood RH. Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis. Pharmacol Res. 2007;55:224–36.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Chan R, Lok K, Woo J. Prostate cancer and vegetable consumption. Mol Nutr Food Res. 2009;53:201–16.

    Article  CAS  PubMed  Google Scholar 

  33. Tang L, Zirpoli GR, Guru K, Moysich KB, Zhang Y, Ambrosone CB, McCann SE. Consumption of raw cruciferous vegetables is inversely associated with bladder cancer risk. Cancer Epidemiol Biomark Prev. 2008;17:938–44.

    Article  CAS  Google Scholar 

  34. Dallas PB, Gottardo NG, Firth MJ, Beesley AH, Hoffmann K, Terry PA, Freitas JR, Boag JM, Cummings AJ, Kees UR. Gene expression levels assessed by oligonucleotide microarray analysis and quantitative real-time RT-PCR—how well do they correlate? BMC Genom. 2005;6:59.

    Article  Google Scholar 

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Acknowledgments

The authors are indebted to the participants for their close cooperation. This study was supported by Shiraz University (Grant Number: 93GRD3M1741).

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Authors and Affiliations

  1. Department of Biology, College of Sciences, Shiraz University, Shiraz, 71467-13565, Iran

    Khyber Saify, Iraj Saadat & Mostafa Saadat

Authors
  1. Khyber Saify
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  2. Iraj Saadat
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  3. Mostafa Saadat
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Correspondence to Mostafa Saadat.

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Saify, K., Saadat, I. & Saadat, M. Influence of A-21T and C-262T genetic polymorphisms at the promoter region of the catalase (CAT) on gene expression. Environ Health Prev Med 21, 382–386 (2016). https://doi.org/10.1007/s12199-016-0540-4

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  • DOI: https://doi.org/10.1007/s12199-016-0540-4

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