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Adipose Stromal Cells are a More Efficient Source than Adipose Stem Cells in Retrovirus-Mediated iPS Induction

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Abstract

Recently, adipose tissue-derived mesenchymal stem cells (MSCs) were reported to be a valuable cell source for generating induced pluripotent stem (iPS) cells because adipose tissue is readily obtainable and possesses high quantities of MSCs. To date, little research has been conducted to characterize the efficiency of iPS induction in adipose tissue. Human iPS cells were generated from adipose tissue-derived MSCs (AT-MSCs) and stromal cells (AT-SCs) via a retroviral delivery system. The reprogramming efficiency of AT-MSCs and AT-SCs were 0.008 and 0.014%, respectively, in terms of the expression of the TRA-1-60 antibody and 0.023 and 0.041%, respectively, regarding alkaline phosphatase staining. This differential reprogramming capability resulted from different levels of viral infectivity between the two cell types. Viral infectivity using pMXs-IRES-GFP was notably low in AT-MSCs and the quantities of OSKM (OCT4, SOX2, KLF4 and c-MYC) in AT-MSCs were 22.5–31.6% of AT-SCs. In conclusion, the amount of transgenes leads to a higher reprogramming efficiency in AT-SCs compared with AT-MSCs. The present study shows that the induction efficiency of pluripotent cells from somatic or adult stem cells could depend on the viral susceptibility of the target cells. Therefore, non-MSCs could be a more efficient source for reprogramming by retrovirus-mediated iPS induction.

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Acknowledgment

This project was supported by research funds from the Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA), Republic of Korea.

Conflicts of interest

Seok-Jin Kang, Young-Il Park, Mi-Jeong Kwon, Yool-Hee Yang, Sa-Ik Bang, Sea-Hwan Sohn, Yong Ho Park, ByungJae So and Hwan-Goo Kang declare that they have no conflicts of interest.

Ethical standards

All human subjects were carried out in accordance with the guidelines of Samsung Medical Center and approved by the hospital’s Institutional Review Board. Also, all animal studies were carried out in accordance with the Code of Laboratory Animal Welfare Ethics, Animal, Plant and Fisheries Quarantine & Inspection Agency (QIA), Republic of Korea.

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Authors and Affiliations

  1. Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency, 480, Anyang, 430-824, Republic of Korea

    Seok-Jin Kang, Young-Il Park, Mi-Jeong Kwon, Yong Ho Park, ByungJae So & Hwan-Goo Kang

  2. Samsung Medical Research Institute, Samsung Medical Center, Seoul, 136-710, Republic of Korea

    Yool-Hee Yang & Sa-Ik Bang

  3. Department of Plastic Surgery, School of Medicine, Sungkyunkwan University, Seoul, 136-710, Republic of Korea

    Sa-Ik Bang

  4. Department of Animal Life Science, Gyeongnam National University of Science and Technology, Jinju, 660-758, Republic of Korea

    Sea-Hwan Sohn

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  1. Seok-Jin Kang
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Associate Editor Michael R. King oversaw the review of this article.

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Kang, SJ., Park, YI., Kwon, MJ. et al. Adipose Stromal Cells are a More Efficient Source than Adipose Stem Cells in Retrovirus-Mediated iPS Induction. Cel. Mol. Bioeng. 8, 224–235 (2015). https://doi.org/10.1007/s12195-014-0374-3

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  • DOI: https://doi.org/10.1007/s12195-014-0374-3

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