Abstract
J proteins are obligate co-chaperones of Hsp70s. Via their signature J domain, all J proteins interact with their partner Hsp70s and stimulate their weak ATPase activity, which is vital for Hsp70 functions. The dependency of J proteins on their J domain is such that mutations in critical amino acids in the J domain often results into a null phenotype for a particular J protein. Here, we show that the J domain of Djp1, a cytosolic J protein important for peroxisomal protein import in Saccharomyces cerevisiae, is partially dispensable. A complete deletion of Djp1 J domain resulted into only partial loss in peroxisomal protein import function. Instead, the C-terminal domain of Djp1 was found to be essential for proper localization of the peroxisomal targeted GFP-PTS1. Furthermore, we show that Caj1, another cytosolic J protein, also has some role in peroxisomal protein import. Caj1 was found to be partially redundant with Djp1 as cells lacking both Djp1 and Caj1 resulted into a much more severe defect in GFP-PTS1 localization. Based on these results, we propose that dispensability of J domains could be attributed to genetic redundancy between different J proteins sharing common structural topology and cellular localization.
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Acknowledgments
We thank Professor Elizabeth Craig (UW-Madison, USA), Dr. B. Distel (Academic Medical Center, The Netherlands), and Dr. Ewald Hettema (University of Sheffield, UK) for yeast strains, plasmids, and antibodies. ND and YT thank DBT and CSIR, New Delhi for fellowships respectively, AKV thanks MHRD for GATE fellowship, PT and SS thank DST for INSPIRE Scholarship. This work was supported by funds from the Department of Biotechnology (BT/PR6885/GBD/27/488/2012 and BT/PR12149/BRB/10/1348/2014), Department of Science and Technology (EMR/2015/001213), and intramural funds from IISER Bhopal.
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Dobriyal, N., Tripathi, P., Sarkar, S. et al. Partial dispensability of Djp1's J domain in peroxisomal protein import in Saccharomyces cerevisiae results from genetic redundancy with another class II J protein, Caj1. Cell Stress and Chaperones 22, 445–452 (2017). https://doi.org/10.1007/s12192-017-0779-8
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DOI: https://doi.org/10.1007/s12192-017-0779-8