Skip to main content
Log in

Partial dispensability of Djp1's J domain in peroxisomal protein import in Saccharomyces cerevisiae results from genetic redundancy with another class II J protein, Caj1

  • Short Communication
  • Published:
Cell Stress and Chaperones Aims and scope

Abstract

J proteins are obligate co-chaperones of Hsp70s. Via their signature J domain, all J proteins interact with their partner Hsp70s and stimulate their weak ATPase activity, which is vital for Hsp70 functions. The dependency of J proteins on their J domain is such that mutations in critical amino acids in the J domain often results into a null phenotype for a particular J protein. Here, we show that the J domain of Djp1, a cytosolic J protein important for peroxisomal protein import in Saccharomyces cerevisiae, is partially dispensable. A complete deletion of Djp1 J domain resulted into only partial loss in peroxisomal protein import function. Instead, the C-terminal domain of Djp1 was found to be essential for proper localization of the peroxisomal targeted GFP-PTS1. Furthermore, we show that Caj1, another cytosolic J protein, also has some role in peroxisomal protein import. Caj1 was found to be partially redundant with Djp1 as cells lacking both Djp1 and Caj1 resulted into a much more severe defect in GFP-PTS1 localization. Based on these results, we propose that dispensability of J domains could be attributed to genetic redundancy between different J proteins sharing common structural topology and cellular localization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

Download references

Acknowledgments

We thank Professor Elizabeth Craig (UW-Madison, USA), Dr. B. Distel (Academic Medical Center, The Netherlands), and Dr. Ewald Hettema (University of Sheffield, UK) for yeast strains, plasmids, and antibodies. ND and YT thank DBT and CSIR, New Delhi for fellowships respectively, AKV thanks MHRD for GATE fellowship, PT and SS thank DST for INSPIRE Scholarship. This work was supported by funds from the Department of Biotechnology (BT/PR6885/GBD/27/488/2012 and BT/PR12149/BRB/10/1348/2014), Department of Science and Technology (EMR/2015/001213), and intramural funds from IISER Bhopal.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Chandan Sahi.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dobriyal, N., Tripathi, P., Sarkar, S. et al. Partial dispensability of Djp1's J domain in peroxisomal protein import in Saccharomyces cerevisiae results from genetic redundancy with another class II J protein, Caj1. Cell Stress and Chaperones 22, 445–452 (2017). https://doi.org/10.1007/s12192-017-0779-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12192-017-0779-8

Keywords

Navigation