Abstract
We aimed to study the relation between plasma levels of stress-induced heat shock protein 70 (HSPA1A) with plasminogen activator inhibitor-1 (PAI-1) and high-density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (Apo-A1), and HDL-C/Apo-A1 ratio. In a matched case-control study on patients with diabetes (40 patients with albuminuria and 40 without albuminuria matched for age, sex, and body mass index), we observed that plasma levels of HSPA1A and PAI-1 are increased in patients with albuminuria (0.55 ± 0.02 vs. 0.77 ± 0.04 ng/ml, p value <0.001 for HSPA1A; 25.9 ± 2 vs. 31.8 ± 2.4 ng/ml, p value <0.05 for PAI-1). There was a significant correlation between HSPA1A and PAI-1 in diabetic patients without albuminuria (r = 0.28; p value = 0.04), but not in those with albuminuria (r = 0.07; p value = 0.63). No association was found between HSPA1A and HDL-C, between HSPA1A and Apo-A1, or between HSPA1A and HDL-C/Apo-A1 ratio. We concluded that there is a direct correlation between plasma HSPA1A and PAI-1 levels in patients with diabetes, which is lost when they develop albuminuria.
References
Abe M, Manola JB, Oh WK, Parslow DL, George DJ, Austin CL, Kantoff PW (2004) Plasma levels of heat shock protein 70 in patients with prostate cancer: a potential biomarker for prostate cancer. Clin Prostate Cancer 3:49–53
Alessi MC, Juhan-Vague I (2004) Contribution of PAI-1 in cardiovascular pathology. Arch Mal Coeur Vaiss 97:673–678
Beckmann RP, Mizzen LE, Welch WJ (1990) Interaction of Hsp 70 with newly synthesized proteins: implications for protein folding and assembly. Science 248:850–854
Brodsky SV, Malinowski K, Golightly M, Jesty J, Goligorsky MS (2002) Plasminogen activator inhibitor-1 promotes formation of endothelial microparticles with procoagulant potential. Circulation 106:2372–2378
Diagnosis and classification of diabetes mellitus (2009) Diabetes Care 32:62–67
Hartl FU, Hayer-Hartl M (2002) Molecular chaperones in the cytosol: from nascent chain to folded protein. Science 295:1852–1858. doi:10.1126/science.1068408
Hirano T, Kashiwazaki K, Moritomo Y, Nagano S, Adachi M (1997) Albuminuria is directly associated with increased plasma PAI-1 and factor VII levels in NIDDM patients. Diabetes Res Clin Pract 36:11–18
Lawrence T, Gilroy DW (2007) Chronic inflammation: a failure of resolution? International Journal of Experimental Pathology 88:85–94. doi:10.1111/j.1365-2613.2006.00507.x
Mansilla MJ, Montalban X, Espejo C (2012) Heat shock protein 70: roles in multiple sclerosis. Mol Med 18:1018–1028. doi:10.2119/molmed.2012.00119
Matsushita K et al (2015) Estimated glomerular filtration rate and albuminuria for prediction of cardiovascular outcomes: a collaborative meta-analysis of individual participant data. Lancet Diabetes Endocrinol 3:514–525. doi:10.1016/S2213-8587(15)00040-6
Mayer MP, Bukau B (2005) Hsp70 chaperones: cellular functions and molecular mechanism. Cell Mol Life Sci 62(6):670–684. doi:10.1007/s00018-004-4464-6
Meng XM, Nikolic-Paterson DJ, Lan HY (2014) Inflammatory processes in renal fibrosis. Nat Nev Nephrol 10(9):493–503. doi:10.1038/nrneph.2014.114
Morteza A, Nakhjavani M, Asgarani F, Ghaneei A, Esteghamati A, Mirmiranpour H (2013a) The lost correlation between leptin and CRP in type 2 diabetes. Eur Cytokine Netw 24:53–59. doi:10.1684/ecn.2013.0329
Morteza A, Nakhjavani M, Larry M, Nargesi AA, Esteghamati A (2013b) Heat shock protein 70 and albuminuria in patients with type 2 diabetes: a matched case control study. Cell Stress Chaperones 18:815–819. doi:10.1007/s12192-013-0435-x
Najafizadeh SR, Ghazizadeh Z, Nargesi AA, Mahdavi M, Abtahi S, Mirmiranpour H, Nakhjavani M (2015) Analysis of serum heat shock protein 70 (HSPA1A) concentrations for diagnosis and disease activity monitoring in patients with rheumatoid arthritis. Cell Stress Chaperones 20:537–543. doi:10.1007/s12192-015-0578-z
Nakhjavani M, Morteza A, Asgarani F, Khalilzadeh O, Ghazizadeh Z, Bathaie SZ, Esteghamati A (2012) The dual behavior of heat shock protein 70 and asymmetric dimethylarginine in relation to serum CRP levels in type 2 diabetes. Gene 498:107–111. doi:10.1016/j.gene.2012.01.085
Nakhjavani M, Morteza A, Nargesi AA, Mostafavi E, Esteghamati A (2015) Appearance of leptin-HSP70 correlation, in type 2 diabetes. Meta Gene 1:1–7. doi:10.1016/j.mgene.2013.09.002
Nargesi AA et al (2014) Manual or automated sphygmomanometer? A historical cohort to quantify measurement bias in blood pressure recording. J Clin Hypertens (Greenwich) 16:716–721. doi:10.1111/jch.12400
Navab M, Reddy ST, Van Lenten BJ, Anantharamaiah GM, Fogelman AM (2009) The role of dysfunctional HDL in atherosclerosis. J Lipid Res 50 Suppl:S145–149 doi:R800036-JLR200 [pii] 10.1194/jlr.R800036-JLR200 [doi]
Njemini R, Demanet C, Mets T (2004) Inflammatory status as an important determinant of heat shock protein 70 serum concentrations during aging. Biogerontology 5(1):31–38
Qian Y, Pennathur S, Kretzler M, Brosius F (2008) From fibrosis to sclerosis; mechanisms of glomerulosclerosis in diabetic nephropathy. Diabetes 57:1439–1445. doi:10.2337/db08-0061
Rizzo M et al (2011) Heat shock protein-60 and risk for cardiovascular disease. Curr Pharm Des 17:3662–3668
Rodrigues-Krause J, Krause M, O'Hagan C, De Vito G, Boreham C, Murphy C, Newsholme P, Colleran G (2012) Divergence of intracellular and extracellular HSP72 in type 2 diabetes: does fat matter? Cell Stress and Chaperones 17(3):293–302. doi:10.1007/s12192-011-0319-x
Ross R (1999) Atherosclerosis—an inflammatory disease. N Engl J Med 340:115–126. doi:10.1056/NEJM199901143400207
Rucker M, Schafer T, Scheuer C, Harder Y, Vollmar B, Menger MD (2006) Local heat shock priming promotes recanalization of thromboembolized microvasculature by upregulation of plasminogen activators. Arterioscler Thromb Vasc Biol 26:1632–1639. doi:10.1161/01.ATV.0000223144.65958.c3
Son SJ et al (2015) Increased plasma levels of heat shock protein 70 associated with subsequent clinical conversion to mild cognitive impairment in cognitively healthy elderly. PLoS One. doi:10.1371/journal.pone.0119180
Stehouwer CD, Gall MA, Twisk JW, Knudsen E, Emeis JJ, Parving HH (2002) Increased urinary albumin excretion, endothelial dysfunction, and chronic low-grade inflammation in type 2 diabetes: progressive, interrelated, and independently associated with risk of death. Diabetes 51:1157–1165
Villeneuve LM, Natarajan R (2010) Epigenetics of diabetic complications. Expert Rev Endocrinol Metab 5:137–148. doi:10.1586/eem.09.54
Zhao R, Shen GX (2007) Involvement of heat shock factor-1 in glycated LDL-induced upregulation of plasminogen activator inhibitor-1 in vascular endothelial cells. Diabetes 56:1436–1444. doi:10.2337/db06-1199
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Nargesi, A.A., Shalchi, M., Nargesi, R.A. et al. The lost correlation between heat shock protein 70 (HSPA1A) and plasminogen activator inhibitor-1 in patients with type 2 diabetes and albuminuria. Cell Stress and Chaperones 21, 361–365 (2016). https://doi.org/10.1007/s12192-015-0634-8
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DOI: https://doi.org/10.1007/s12192-015-0634-8