Abstract
We previously found that endoplasmic reticulum stress (ERS) might be exhibited in the conventional protocol of the primary culture of neonate rat myocardial cells (NRMCs) and that the high glucose concentration (25 mmol/L) in the culture medium might be the cause. Here, we investigated if the high concentration of glucose might influence ERS in myocardial cells during culture. GRP78 expression (ERS marker) was similar in groups with tunicamycin (TM) and without TM in high glucose cultured cells (p > 0.01). Different glucose concentrations elicited different GRP78 expressions according to analyses of protein and RNA levels, which showed ERS in H/H groups. Finally, we found that GRP78 expression was higher in TM groups compared with M/M groups (p < 0.01). The conventional high-glucose culture media during primary culture of NRMCs induced ERS. We propose that medium-glucose culture media should be used and describe an improved protocol for the primary culture of NRMCs.
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Acknowledgments
This study was supported by the National Science Foundation for Young Scholars of China (grant number 30900612), the Grant of Medical Science Research Foundation of Zhejiang Province (grant number 2009B127), and from the Zhejiang Provincial Natural Science Foundation (grant number Y2090393), People’s Republic of China.
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Wang, Q., Jin, J., Guo, Z. et al. Improvements in the primary culture of neonate rat myocardial cells by study of the mechanism of endoplasmic reticulum stress. Cell Stress and Chaperones 18, 367–375 (2013). https://doi.org/10.1007/s12192-012-0390-y
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DOI: https://doi.org/10.1007/s12192-012-0390-y