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Functional relevance of J-protein family of rice (Oryza sativa)

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Cell Stress and Chaperones Aims and scope

Abstract

Protein folding and disaggregation are crucial processes for survival of cells under unfavorable conditions. A network of molecular chaperones supports these processes. Collaborative action of Hsp70 and Hsp100 proteins is an important component of this network. J-proteins/DnaJ members as co-chaperones assist Hsp70. As against 22 DnaJ sequences noted in yeast, rice genome contains 104 J-genes. Rice J-genes were systematically classified into type A (12 sequences), type B (9 sequences), and type C (83 sequences) classes and a scheme of nomenclature of these proteins is proposed. Transcript expression profiles revealed that J-proteins are possibly involved in basal cellular activities, developmental programs, and in stress. Ydj1 is the most abundant J-protein in yeast. Ydj1 deleted yeast cells are nonviable at 37 °C. Two rice ortholog proteins of yeast Ydj1 protein namely OsDjA4 and OsDjA5 successfully rescued the growth defect in mutant yeast. As Hsp70 and J-proteins work in conjunction, it emerges that rice J-proteins can partner with yeast Hsp70 proteins in functioning. It is thus shown that J-protein machine is highly conserved.

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Acknowledgement

We thank Jeffrey L. Brodsky of the University of Pittsburgh, USA for DnaJ yeast mutants and Hao Yu of the National University of Singapore, Singapore for kindly providing the seeds of T-DNA insertion mutants of Arabidopsis J3 protein (j3-1 and j3-2). We thank Centre for Plant Molecular Biology and Indo-Finland project, Department of Biotechnology, Govt. of India, for financial support.

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Authors and Affiliations

  1. Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India

    Neelam K Sarkar, Upasna Thapar, Preeti Kundnani, Priyankar Panwar & Anil Grover

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  1. Neelam K Sarkar
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  2. Upasna Thapar
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  3. Preeti Kundnani
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  4. Priyankar Panwar
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Correspondence to Anil Grover.

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ESM Fig. 1

FGENESH analysis of Os03g12236 (PDF 27 kb)

ESM Fig. 2

Multiple sequence alignment of J-domain of rice J-proteins showing consensus sequences and four helices characteristic of J-domain. The sequences were aligned in Clustal and edited in Jalview. The consensus sequence and the Logo were derived using Jalview. The amino acid residues are colored by default color scheme of ClustalX. Conserved tripeptide HPD is present between helix II and III. J-proteins in which HPD tripeptide was absent are depicted in red color font (EPS 4,581 kb)

ESM Fig. 3

Tandem arrangement of J-proteins on chromosome (PPTX 387 kb)

ESM Fig. 4

Microarray based expression meta-analysis of type C J-genes in abiotic stresses (a), types C and D during development stages of rice plant (b) and in various tissues (c) (PPTX 346 kb)

ESM Fig. 5

Microarray based expression meta-analysis of types A and B J-genes in development stages (a) and various tissues of rice plant (b) (PPTX 175 kb)

ESM Table 1

Prediction of localization of J-proteins of rice (XLSX 16 kb)

ESM Table 2

List of primers used in this study (XLSX 10 kb)

ESM Table 3

Details of J-proteins of rice without HPD tripeptide in J-domain (XLSX 12.2 kb)

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Sarkar, N.K., Thapar, U., Kundnani, P. et al. Functional relevance of J-protein family of rice (Oryza sativa). Cell Stress and Chaperones 18, 321–331 (2013). https://doi.org/10.1007/s12192-012-0384-9

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  • DOI: https://doi.org/10.1007/s12192-012-0384-9

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