Skip to main content
SpringerLink
Log in

Changes in anti-heat shock protein 27 antibody and C-reactive protein levels following cardiac surgery and their association with cardiac function in patients with cardiovascular disease

  • Original Paper
  • Published:
Cell Stress and Chaperones Aims and scope

Abstract

The relationship between serum anti-heat shock protein (Hsp)27 antibody and high sensitive C-reactive protein (hs-CRP) levels and indices of cardiac function were investigated in patients undergoing coronary artery bypass grafting (CABG) or heart valve replacement. The changes in anti-Hsp27 antibody titers and hs-CRP levels were compared among patients undergoing off-pump and on-pump CABG or valvular heart replacement. Fifty-three patients underwent off-pump, on-pump CABG, and heart valvular replacement in each group. Serum anti-Hsp27 titers and hs-CRP values were measured 24 h before and after the operation and at discharge. Echocardiography was performed before surgery and before discharge. The results were compared with values from 83 healthy controls. hs-CRP levels increased and anti-Hsp27 antibody decreased following surgery (P < 0.001 and P < 0.05, respectively), although these changes were independent of operative procedure (P = 0.361 and P = 0.120, respectively). Anti-Hsp27 antibody levels were higher at the time of discharge (P = 0.016). Only in coronary patients were anti-Hsp27 antibody levels negatively associated with E/E′ (r = −0.268, P = 0.022), a marker of pulmonary capillary wedge pressure. In conclusions, anti-Hsp27 antibody levels are associated with indices of cardiac function in coronary patients. Cardiopulmonary bypass had no significant effect on the induction of changes in anti-Hsp27 levels. Moreover, anti-Hsp27 antibody levels fell in all groups postoperatively; this may be due to the formation of immune complexes of antigen–antibody, and antibody levels were higher at the time of discharge.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Ascione R, Lloyd CT, Underwood MJ et al (2000) Inflammatory response after coronary revascularization with or without cardiopulmonary bypass. Ann Thorac Surg 69:1198–1204

    Article  PubMed  CAS  Google Scholar 

  • Atar I, Gulmez O, Atar A et al (2005) The effects of prior beta-blocker therapy on serum C-reactive protein levels after percutaneous coronary intervention. Clin Cardiol 28:243–246

    Article  PubMed  Google Scholar 

  • Beatty WL, Morrison RP, Byrne GI (1994) Persistent chlamydiae: from cell culture to a paradigm for chlamydial pathogenesis. Microbiol Rev 58:686–699

    PubMed  CAS  Google Scholar 

  • Biglioli P, Cannata A, Alamanni F et al (2003) Biological effects of off-pump vs. on-pump coronary artery surgery: focus on inflammation, hemostasis and oxidative stress. Eur J Cardiothorac Surg 24:260–269

    Article  PubMed  Google Scholar 

  • Bolli R (1991) Oxygen-derived free radicals and myocardial reperfusion injury: an overview. Cardiovasc Drugs Ther 5(Suppl 2):249–268

    Article  PubMed  Google Scholar 

  • Boulanger CM, Scoazec A, Ebrahimian T et al (2001) Circulating microparticles from patients with myocardial infarction cause endothelial dysfunction. Circulation 104:2649–2652

    Article  PubMed  CAS  Google Scholar 

  • Burke AP, Tracy RP, Kolodgie F et al (2002) Elevated C-reactive protein values and atherosclerosis in sudden coronary death: association with different pathologies. Circulation 105:2019–2023

    Article  PubMed  CAS  Google Scholar 

  • Clements RT, Sodha NR, Feng J et al (2007) Phosphorylation and translocation of heat shock protein 27 and alphaB-crystallin in human myocardium after cardioplegia and cardiopulmonary bypass. J Thorac Cardiovasc Surg 134:1461–1470

    Article  PubMed  CAS  Google Scholar 

  • Cleveland JC Jr, Shroyer AL, Chen AY, Peterson E, Grover FL (2001) Off-pump coronary artery bypass grafting decreases risk-adjusted mortality and morbidity. Ann Thorac Surg 72:1282–1288

    Article  PubMed  Google Scholar 

  • Davutoglu V, Celik A, Aksoy M (2005) Contribution of selected serum inflammatory mediators to the progression of chronic rheumatic valve disease, subsequent valve calcification and NYHA functional class. J Heart Valve Dis 14:251–256

    PubMed  Google Scholar 

  • Eberhardt F, Mehlhorn U, Larose K, De Vivie ER, Dhein S (2000) Structural myocardial changes after coronary artery surgery. Eur J Clin Invest 30:938–946

    PubMed  CAS  Google Scholar 

  • Fransen E, Maessen J, Dentener M et al (1998) Systemic inflammation present in patients undergoing CABG without extracorporeal circulation. Chest 113:1290–1295

    Article  PubMed  CAS  Google Scholar 

  • Garcia MJ, Thomas JD, Klein AL (1998) New Doppler echocardiographic applications for the study of diastolic function. J Am Coll Cardiol 32:865–875

    Article  PubMed  CAS  Google Scholar 

  • Gaspardone A, Crea F, Versaci F et al (1998) Predictive value of C-reactive protein after successful coronary-artery stenting in patients with stable angina. Am J Cardiol 82:515–518

    Article  PubMed  CAS  Google Scholar 

  • Ghayour-Mobarhan M, Sahebkar A, Parizadeh SM et al (2008) Antibody titres to heat shock protein 27 are elevated in patients with acute coronary syndrome. Int J Exp Pathol 89:209–215

    Article  PubMed  CAS  Google Scholar 

  • Ghayour-Mobarhan M, Rahsepar AA, Tavallaie S, Rahsepar S, Ferns GA (2009) The potential role of heat shock proteins in cardiovascular disease: evidence from in vitro and in vivo studies. Adv Clin Chem 48:27–72

    Article  PubMed  CAS  Google Scholar 

  • Giannessi D, Colotti C, Maltinti M et al (2007) Circulating heat shock proteins and inflammatory markers in patients with idiopathic left ventricular dysfunction: their relationships with myocardial and microvascular impairment. Cell Stress Chaperones 12:265–274

    Article  PubMed  CAS  Google Scholar 

  • Gottsauner-Wolf M, Zasmeta G, Hornykewycz S et al (2000) Plasma levels of C-reactive protein after coronary stent implantation. Eur Heart J 21:1152–1158

    Article  PubMed  CAS  Google Scholar 

  • Hearse DJ (1990) Ischemia, reperfusion, and the determinants of tissue injury. Cardiovasc Drugs Ther 4(Suppl 4):767–776

    Article  PubMed  Google Scholar 

  • Heidari-Bakavoli AR, Sahebkar A, Mobara N et al (2012) Changes in plasma level of heat shock protein 27 after acute coronary syndrome. Angiology 63:12–16

    Article  PubMed  CAS  Google Scholar 

  • Karu I, Taal G, Zilmer K et al (2010) Inflammatory/oxidative stress during the first week after different types of cardiac surgery. Scand Cardiovasc J 44:119–124

    Article  PubMed  CAS  Google Scholar 

  • Kazemi-Bajestani SM, Ghayour-Mobarhan M, Ebrahimi M et al (2007) C-reactive protein associated with coronary artery disease in Iranian patients with angiographically defined coronary artery disease. Clin Lab 53:49–56

    PubMed  CAS  Google Scholar 

  • Lindquist S (1986) The heat-shock response. Annu Rev Biochem 55:1151–1191

    Article  PubMed  CAS  Google Scholar 

  • Mandal K, Jahangiri M, Xu Q (2005) Autoimmune mechanisms of atherosclerosis. Handb Exp Pharmacol 170:723–743

    Google Scholar 

  • Martin JL, Mestril R, Hilal-Dandan R, Brunton LL, Dillmann WH (1997) Small heat shock proteins and protection against ischemic injury in cardiac myocytes. Circulation 96:4343–4348

    Article  PubMed  CAS  Google Scholar 

  • Martin JL, Hickey E, Weber LA, Dillmann WH, Mestril R (1999) Influence of phosphorylation and oligomerization on the protective role of the small heat shock protein 27 in rat adult cardiomyocytes. Gene Expr 7:349–355

    PubMed  CAS  Google Scholar 

  • Miller JD, Weiss RM, Serrano KM et al (2009) Lowering plasma cholesterol levels halts progression of aortic valve disease in mice. Circulation 119:2693–2701

    Article  PubMed  CAS  Google Scholar 

  • Moohebati M, Falsoleiman H, Dehghani M et al (2011) Serum inflammatory and immune marker response after bare-metal or drug-eluting stent implantation following percutaneous coronary intervention. Angiology 62:184–190

    Article  PubMed  CAS  Google Scholar 

  • O’Brien KD (2006) Pathogenesis of calcific aortic valve disease: a disease process comes of age (and a good deal more). Arterioscler Thromb Vasc Biol 26:1721–1728

    Article  PubMed  Google Scholar 

  • Pourghadamyari H, Moohebati M, Parizadeh SM et al (2011) Serum antibody titers against heat shock protein 27 are associated with the severity of coronary artery disease. Cell Stress Chaperones 16:309–316

    Article  PubMed  CAS  Google Scholar 

  • Rahsepar AA, Mirzaee A, Moodi F et al. (2012) Anti-heat shock protein 27 titers and oxidative stress levels are elevated in patients with valvular heart disease. Angiology. doi:10.1177/0003319711433308

  • Rastan AJ, Eckenstein JI, Hentschel B et al (2006) Emergency coronary artery bypass graft surgery for acute coronary syndrome: beating heart versus conventional cardioplegic cardiac arrest strategies. Circulation 114:I477–I485

    Article  PubMed  Google Scholar 

  • Sabik JF, Gillinov AM, Blackstone EH et al (2002) Does off-pump coronary surgery reduce morbidity and mortality? J Thorac Cardiovasc Surg 124:698–707

    Article  PubMed  Google Scholar 

  • Schett G, Metzler B, Kleindienst R et al (1999) Myocardial injury leads to a release of heat shock protein (hsp) 60 and a suppression of the anti-hsp65 immune response. Cardiovasc Res 42:685–695

    Article  PubMed  CAS  Google Scholar 

  • Stollerman GH (1997) Rheumatic fever. Lancet 349:935–942

    Article  PubMed  CAS  Google Scholar 

  • Szerafin T, Horvath A, Moser B et al (2006) Apoptosis-specific activation markers in on- versus off-pump coronary artery bypass graft (CABG) patients. Clin Lab 52:255–261

    PubMed  Google Scholar 

  • Vaage J, Valen G (1993) Pathophysiology and mediators of ischemia–reperfusion injury with special reference to cardiac surgery. A review. Scand J Thorac Cardiovasc Surg Suppl 41:1–18

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are particularly grateful to the patients and their family members who volunteered to participate in this study. This work was supported by Mashhad University of Medical Science (MUMS), Iran. The results presented in this work have been taken from Amir Ali Rahsepar’s thesis in MUMS, with the following ID number: 87065.

Conflict of interest

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. Cardiovascular Research Center, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran

    Amir Ali Rahsepar, Asadollah Mirzaee, Fatemeh Moodi, Mohsen Moohebati, Fatemeh Khorashadizadeh, Ali Eshraghi, Maryam-Sadat Alavi, Laya Zarrabi, Mostafa Sajjadian, Maral Amini, Roghayeh Paydar, Somayeh Mousavi & Majid Ghayour-Mobarhan

  2. Biochemistry of Nutrition Research Center, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran

    Amir Ali Rahsepar, Shima Tavallaie, Roshanak Khojasteh & Majid Ghayour-Mobarhan

  3. Department of Cardiovascular Surgery, Quem Hospital, Mashhad University of Medical Science, Mashhad, Iran

    Asadollah Mirzaee

  4. Institute for Science & Technology in Medicine, Thornburrow Drive, University of Keele, Stoke on Trent, Staffordshire, ST4 7QB, UK

    Gordon A. Ferns

Authors
  1. Amir Ali Rahsepar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Asadollah Mirzaee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fatemeh Moodi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohsen Moohebati
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shima Tavallaie
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fatemeh Khorashadizadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ali Eshraghi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Maryam-Sadat Alavi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Laya Zarrabi
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Mostafa Sajjadian
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Maral Amini
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Roshanak Khojasteh
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Roghayeh Paydar
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Somayeh Mousavi
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Majid Ghayour-Mobarhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. Gordon A. Ferns
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Majid Ghayour-Mobarhan.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(PDF 61.9 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rahsepar, A.A., Mirzaee, A., Moodi, F. et al. Changes in anti-heat shock protein 27 antibody and C-reactive protein levels following cardiac surgery and their association with cardiac function in patients with cardiovascular disease. Cell Stress and Chaperones 18, 65–74 (2013). https://doi.org/10.1007/s12192-012-0358-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12192-012-0358-y

Keywords

Search

Navigation