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Hsp60 and Hsp10 increase in colon mucosa of Crohn’s disease and ulcerative colitis

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Cell Stress and Chaperones Aims and scope

Abstract

The purpose of this work was to determine in colon mucosa of Crohn’s disease (CD) and ulcerative colitis (UC) in relapse: a) the levels of the chaperonins Hsp60 and Hsp10; b) the quantity of inflammatory cells; and c) if the levels of chaperonins parallel those of inflammation cells. Twenty cases of CD and UC and twenty normal controls (NC) were studied using immunohistochemistry, Western blotting and immunofluorescence. Immunohistochemically, Hsp60 and Hsp10 were increased in both inflammatory bowel diseases (IBD) compared to NC. These results were confirmed by Western blotting. Hsp60 and Hsp10 occurred in the cytoplasm of epithelial cells in CD and UC but not in NC. Hsp60 and Hsp10 co-localised to epithelial cells of mucosal glands but not always in connective tissue cells of lamina propria, where only Hsp60 or, less often, Hsp10 was found. Cells typical of inflammation were significantly more abundant in CD and UC than in NC. Since chaperonins are key factors in the activation of the immune system leading to inflammation, we propose that they play a central role in the pathogenesis of the two diseases, which, consequently, ought to be studied as chaperonopathies.

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Acknowledgements

This work was supported by funds of University of Palermo (VR, SD, GZ and FC) and Istituto Euro-Mediterraneo di Scienza e Tecnologia (FC).

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Authors and Affiliations

  1. Dipartimento di Patologia Umana, Università degli Studi di Palermo, Palermo, Italy

    Vito Rodolico, Monica Zerilli & Anna Martorana

  2. Dipartimento di Chirurgia Generale, d’Urgenza e Trapianti d’Organo, Università degli Studi di Palermo, Palermo, Italy

    Giovanni Tomasello

  3. Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Università degli Studi di Palermo, Sezione di Anatomia Umana, via del Vespro 129, 90127, Palermo, Italy

    Alessandro Pitruzzella, Antonella Marino Gammazza, Sabrina David, Giovanni Zummo & Francesco Cappello

  4. Dipartimento di Medicina Interna, Malattie Cardiovascolari e Nefrourologiche, Università degli Studi di Palermo, Palermo, Italy

    Provvidenza Damiani

  5. Dipartimento Materno-Infantile, Università degli Studi di Palermo, Palermo, Italy

    Salvatore Accomando

  6. University of Maryland, IMET, Baltimore, MD, USA

    Everly Conway de Macario & Alberto J. L. Macario

  7. Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy

    Francesco Cappello

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  1. Vito Rodolico
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  2. Giovanni Tomasello
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  3. Monica Zerilli
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  12. Alberto J. L. Macario
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Correspondence to Francesco Cappello.

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Vito Rodolico and Giovanni Tomasello contributed equally to the present work.

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Rodolico, V., Tomasello, G., Zerilli, M. et al. Hsp60 and Hsp10 increase in colon mucosa of Crohn’s disease and ulcerative colitis. Cell Stress and Chaperones 15, 877–884 (2010). https://doi.org/10.1007/s12192-010-0196-8

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  • DOI: https://doi.org/10.1007/s12192-010-0196-8

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