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Isolation of mesenchymal stromal/stem cells from small-volume umbilical cord blood units that do not qualify for the banking system

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Abstract

The clinical application of mesenchymal stromal/stem cells (MSCs) has been extensively explored. In this study, we examined the availability of freshly donated umbilical cord blood (UCB) units that do not qualify for the Japanese banking system for transplantation because of their small volume as a source of MSCs. Forty-five UCB units were used. The median volume of each UCB unit and number of nucleated cells per unit were 40 mL and 5.39 × 108, respectively. MSCs were successfully isolated from 18 of 45 units (40 %). The MSC isolation rate was not affected by cell processing method or the interval between delivery and cell processing. The volume of the UCB unit and the mononuclear cell count were predictive factors of the MSC isolation rate. MSCs were effectively isolated by selecting UCB units with a volume of ≥54 mL and containing ≥1.28 × 108 mononuclear cells, yielding a MSC isolation rate of >70 %. UCB-derived MSCs were similar to bone marrow-derived MSCs in terms of their morphology, surface marker expression, and differentiation potential, apart from adipogenesis. Our data indicate that UCB units that are currently discarded due to inadequate volume should be reconsidered as a source of MSCs using the well-established UCB banking system.

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Acknowledgments

We thank all the mothers who allowed us to use their UCB in this study. We also thank Dr. Yayoi Higashi, Ms. Mieko Takumi, and the nursing staff of A7 ward in Japanese Red Cross Kyoto Daini Hospital for coordinating UCB donation from the Japanese Red Cross Kinki Block Blood Center. This work was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology in Japan (Y.M., T.I., H.H., M.M., A.T-K., and T.M.) and a Grant-in-Aid from the Ministry of Health, Labour, and Welfare in Japan (T.I. and T.M.). This work was also supported in part by the Joint Usage/Research Center of Hiroshima University Research Institute for Radiation Biology and Medicine (Y.M. and T.I.), the Cell Science Research Foundation (Y.M.), and the Takeda Science Foundation (Y.M.).

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Authors and Affiliations

  1. Department of Hematology, Kobe City Medical Center General Hospital, Kobe, 650-0046, Japan

    Satoshi Yoshioka

  2. Department of Hematology/Oncology, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan

    Satoshi Yoshioka & Akifumi Takaori-Kondo

  3. Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, 54 Kawaharacho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan

    Satoshi Yoshioka, Yasuo Miura, Masaki Iwasa, Aya Fujishiro, Hisayuki Yao, Yoko Nakagawa, Asumi Yokota, Hideyo Hirai & Taira Maekawa

  4. Division of Gastroenterology and Hematology, Shiga University of Medical Science, Otsu, Shiga, 520-2192, Japan

    Masaki Iwasa & Aya Fujishiro

  5. Department of Endocrinology, Rakuwakai Otowa Hospital, Kyoto, 607-8062, Japan

    Masako Miura

  6. Department of Obstetrics and Gynecology, Japanese Red Cross Kyoto Daini Hospital, Kyoto, 602-8026, Japan

    Masaaki Fukuoka

  7. Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, 734-8553, Japan

    Tatsuo Ichinohe

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  1. Satoshi Yoshioka
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  2. Yasuo Miura
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  3. Masaki Iwasa
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  5. Hisayuki Yao
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  6. Masako Miura
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  7. Masaaki Fukuoka
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  9. Asumi Yokota
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  10. Hideyo Hirai
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Correspondence to Yasuo Miura.

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Yoshioka, S., Miura, Y., Iwasa, M. et al. Isolation of mesenchymal stromal/stem cells from small-volume umbilical cord blood units that do not qualify for the banking system. Int J Hematol 102, 218–229 (2015). https://doi.org/10.1007/s12185-015-1828-7

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  • DOI: https://doi.org/10.1007/s12185-015-1828-7

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