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Evaluation of HNA-expressing cell line-based antigen capture systems and a solid-phase system for detecting HNA-1a antibodies

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Abstract

Granulocyte immunofluorescence and granulocyte agglutination tests are standard methods for detecting human neutrophil antigen (HNA) antibodies (Abs); however, these require a typed panel of neutrophils, which can be time-consuming to develop, and it remains difficult to determine antibody specificity in some cases. We established and evaluated four detection systems for HNA-1a Abs based on an HNA-1a-expressing cell line (KY cells) and antigen capture. We additionally evaluated a commercial solid-phase system. Eleven HNA-1a antibody-positive samples, including the World Health Organization Reference Reagent, and 40 serum samples derived from male blood donors were used as positive and negative control samples, respectively. Although specificity was >0.90 in all systems evaluated, the sensitivity varied among the systems. The KY cell-based monoclonal antibody specific immobilisation of granulocyte antigens (KY-MAIGA) system using certain, but not all, monoclonal Abs, and the solid-phase system revealed higher sensitivity than other systems. In conclusion, the KY-MAIGA and commercial solid-phase systems were superior in terms of specific and sensitive detection of HNA-1a Abs.

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Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Japanese Red Cross Kinki Block Blood Center, 7-5-17, Saitoasagi, Ibaraki, Osaka, 567-0085, Japan

    Etsuko Amakishi, Yoriko Irie, Kanae Nishizawa, Nobuki Matsuyama, Hiroyuki Ishii, Harumichi Matsukura, Kazuta Yasui & Fumiya Hirayama

  2. Central Blood Institute, Japanese Red Cross Society, Tokyo, Japan

    Hiromi Kamada & Fumiaki Nakajima

Authors
  1. Etsuko Amakishi
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  2. Yoriko Irie
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  3. Kanae Nishizawa
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  4. Hiromi Kamada
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  5. Fumiaki Nakajima
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  6. Nobuki Matsuyama
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  7. Hiroyuki Ishii
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  8. Harumichi Matsukura
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  9. Kazuta Yasui
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  10. Fumiya Hirayama
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Corresponding author

Correspondence to Fumiya Hirayama.

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Amakishi, E., Irie, Y., Nishizawa, K. et al. Evaluation of HNA-expressing cell line-based antigen capture systems and a solid-phase system for detecting HNA-1a antibodies. Int J Hematol 101, 386–391 (2015). https://doi.org/10.1007/s12185-015-1751-y

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  • DOI: https://doi.org/10.1007/s12185-015-1751-y

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