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A Novel Staphylococcal Enterotoxin Q Immunosensor Prepared with Self-Assembly Method Based on Horseradish Peroxidase and Double-Layer Gold Nanoparticles

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Abstract

A novel and green electrochemical immunosensor for detection of Staphylococcal enterotoxin Q (SEQ), a toxic superantigen that can induce severe food poisoning and even fatal conditions, was developed by fixing double-layer gold nanoparticles (AuNPs), horseradish peroxidase, and thionin-chitosan composite membrane on the glassy carbon electrode surface. Under optimal conditions, the developed immunosensor showed a wide linear range from 0.1 to 100 pg mL−1 (R 2 = 0.992) for SEQ with a low detection limit of 0.046 pg mL−1 (S/N = 3). The immunosensor had good specificity (no significant cross reaction with lipopolysaccharides, bovine IgG, Escherichia coli, or other common biological components), and remained fairly stable (over 87 % of the original signal response after stored for 20 days at 4 °C). In addition, the immunosensor was successfully applied to milk sample detection and demonstrated with high recoveries (from 91 to 113 %). In conclusion, the developed electrochemical immunosensor can supply a green and feasible tool for detection of SEQ in food.

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Acknowledgments

The work was supported by the National Natural Science Foundation of China (No. 31371773).

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Authors and Affiliations

  1. Biotechnology and Food Science College, Tianjin University of Commerce, Tianjin, 300134, People’s Republic of China

    Lihui Wei, Xingya Wang, Dingqiang Lu, Yang Li, Guangchang Pang & Junbo Xie

  2. Tianjin Key Laboratory of Food Biotechnology, Tianjin, 300134, People’s Republic of China

    Guangchang Pang & Junbo Xie

Authors
  1. Lihui Wei
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  2. Xingya Wang
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  3. Dingqiang Lu
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  4. Yang Li
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  5. Guangchang Pang
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  6. Junbo Xie
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Corresponding authors

Correspondence to Guangchang Pang or Junbo Xie.

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Funding

The study was funded by the National Natural Science Foundation of China (grant number 31371773).

Conflict of Interest

The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Not applicable.

Additional information

Lihui Wei and Xingya Wang contributed equally to this work.

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Wei, L., Wang, X., Lu, D. et al. A Novel Staphylococcal Enterotoxin Q Immunosensor Prepared with Self-Assembly Method Based on Horseradish Peroxidase and Double-Layer Gold Nanoparticles. Food Anal. Methods 10, 892–899 (2017). https://doi.org/10.1007/s12161-016-0632-1

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  • DOI: https://doi.org/10.1007/s12161-016-0632-1

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