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A Low-Cost and High Sensitive Paper-Based Microfluidic Device for Rapid Detection of Glucose in Fruit

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Abstract

The concentration of glucose in fruit is a key index for fruit quality. Despite the fact that there are many methods to detect glucose, finding a rapid, visual, and inexpensive method to detect glucose in fruit is still a great challenge. In this study, the concept of paper-based microfluidic device (μPAD) was extended to high-performance chemo-sensors for semi-quantitative detection of glucose in different kinds of fruit. Under optimized conditions, the results showed that the method detected glucose of different concentrations and the limit of detection (LOD) can reach 3.12 mM. In the quantifications, a good linear relationship was obtained between mean intensity and glucose concentration (5∼50 mM, R2 = 0.952). The analytical results obtained by the developed approach were in good agreement with the results by spectrophotometric method. In addition, polyethylene glycol (PEG) was added to cellulose paper surface to improve the both hydrophilic and sensing performance, indicating that the PEG-cellulose paper was superior for its rapid response for glucose. Herein, a low-cost and high sensitive μPAD sensor was exploited to assess the fruit quality and guide fruit intake for diabetes mellitus patients.

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Acknowledgments

This study was funded by the National Natural Science Foundation of China (31271870, 31501555), the project of Science and Technology Commission of Shanghai Municipality (14DZ1205100, 14ZR1416600, 14PJ1404300), Key Project of Shanghai Agriculture Prosperity through Science and Technology (2014, 3-5 and 2015, 4-8), Special Foundation for Science and Technology Development of Shanghai Ocean University (A2-0209-15-200006, A2-0302-14-300075). This work was also supported by the program granted by Funding program for outstanding dissertations of Shanghai Ocean University.

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Authors and Affiliations

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China

    Zexian Li, Yongheng Zhu, Weijia Zhang, Changhua Xu, Yingjie Pan & Yong Zhao

  2. Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai, 201306, China

    Zexian Li, Yongheng Zhu, Weijia Zhang, Changhua Xu, Yingjie Pan & Yong Zhao

  3. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai, 201306, China

    Zexian Li, Yongheng Zhu, Weijia Zhang, Changhua Xu, Yingjie Pan & Yong Zhao

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  1. Zexian Li
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  2. Yongheng Zhu
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  3. Weijia Zhang
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  4. Changhua Xu
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  5. Yingjie Pan
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  6. Yong Zhao
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Correspondence to Yongheng Zhu or Yong Zhao.

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This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of Interest

Zexian Li declares that he has no conflict of interest. Yongheng Zhu declares that he has no conflict of interest. Weijia Zhang declares that he has no conflict of interest. Changhua Xu declares that he has no conflict of interest. Yingjie Pan declares that he has no conflict of interest. Yong Zhao declares that he has no conflict of interest.

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Li, Z., Zhu, Y., Zhang, W. et al. A Low-Cost and High Sensitive Paper-Based Microfluidic Device for Rapid Detection of Glucose in Fruit. Food Anal. Methods 10, 666–674 (2017). https://doi.org/10.1007/s12161-016-0626-z

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  • DOI: https://doi.org/10.1007/s12161-016-0626-z

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