Abstract
A new method was developed for the determination of four acrylamide compounds (acrylamide, methacrylamide, N-methylol acrylamide, N-(Methoxymethyl)methacrylamide) in food contact paper products, three kinds of water-based food simulants, and dry food simulant (modified polyphenylene oxide, MPPO) by using ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Acetonitrile was used as the extraction solvent for different kinds of samples. The extraction solution of paper products was purified with QuEChERS technology. Four analytes were separated by gradient elution in a UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm) with methanol and 0.1 % formic acid water as mobile phases, and then detected in electrospray ionization mode of MS/MS with multiple reaction monitoring (MRM). Under the optimal conditions, the calibration curves for four analytes were linear within the range of 1.0–200 μg/L and the correlation coefficients were higher than 0.998. The quantitation limits of the method (S/N = 10) of four analytes were in the range of 0.3–20 μg/kg. The mean recoveries for five sample matrixes at three spiked concentration levels of 0.3–200 μg/kg were in the range of 81–108 % with the relative standard deviations (RSDs, n = 6) values ranging from 2.5 to 7.1 %. The developed method is accurate, simple and rapid, which can be applied to the determination of acrylamide compounds in food contact paper products and food simulants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albishri HM, El-Hady DA (2014) Eco-friendly ionic liquid based ultrasonic assisted selective extraction coupled with a simple liquid chromatography for the reliable determination of acrylamide in food samples. Talanta 118:129–136
Backe WJ, Yingling V, Johnson T (2014) The determination of acrylamide in environmental and drinking waters by large-volume injection-hydrophilic-interaction liquid chromatography and tandem mass spectrometry. J Chromatogr A 1334:72–78
Bent GA, Maragh P, Dasgupta T (2012) Acrylamide in Caribbean foods-residual levels and their relation to reducing sugar and asparagine content. Food Chem 133:451–457
Dong H, Guo X, Xian Y, Luo H, Wang B, Wu Y (2015) A salting out-acetonitrile homogeneous extraction coupled with gas chromatography-mass spectrometry method for the simultaneous determination of thirteen N-nitrosamines in skin care cosmetics. J Chromatogr A 1422:82–88
EN 14338–2003 (2003) Paper and board intended to come into contact with foodstuffs-Conditions for determination of migration from paper and board using modified polyphenylene oxide (MPPO) as a stimulant, European Committee for Standardization
GB 9685–2008 (2008) Hygienic standards for uses of additives in food containers and packaging materials, National Standard of the People’s Republic of China
GB/T 23296.1-2009 (2009) Materials and articles in contact with foodstuffs-Plastics substances subject to limitation-Guide to test methods for the specific migration of substances from plastics to foods and food simulants and the determination of substances in plastics and the selection of conditions of exposure to food simulants, National Standard of the People’s Republic of China
GB/T 23296.9-2009 (2009) Food contact materials-polymer-Determination of acrylamide in food stimulants-High performance liquid chromatography, National Standard of the People’s Republic of China
Guo X et al (2013) Quantitative determinations of seven fluorescent whitening agents in polystyrene and polyvinyl chloride plastics by ultrahigh performance liquid chromatography-tandem mass spectrometry. Anal Methods 5:6086–6093
IARC (1994) Acrylamide. Monographs on the evaluation of carcinogenic risks to humans: some industrial chemicals (Vol. 60, pp. 389–433). Lyon, France
Kaplan O, Kaya G, Ozcan C, Ince M, Yaman M (2009) Acrylamide concentrations in grilled foodstuffs of Turkish kitchen by high performance liquid chromatography-mass spectrometry. Microchem J 93:173–179
Karasek L, Wenzl T, Anklam E (2009) Determination of acrylamide in roasted chestnuts and chestnut-based foods by isotope dilution HPLC-MS/MS. Food Chem 114:1555–1558
Kim TH et al (2015) Determination of acrylamide and glycidamide in various biological matrices by liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study. Talanta 131:46–54
Krska R et al (2012) Challenges and trends in the determination of selected chemical contaminants and allergens in food. Anal Bioanal Chem 402(1):139–162
Liu J, Zhao G, Yuan Y, Chen F, Hu X (2008) Quantitative analysis of acrylamide in tea by liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Food Chem 108:760–767
Michalak J, Gujska E, Kuncewicz A (2013) RP-HPLC-DAD studies on acrylamide in cereal-based baby foods. J Food Compos Anal 32:68–73
Mo W, He H, Xu X, Huang B, Ren Y (2014) Simultaneous determination of ethyl carbamate, chloropropanols and acrylamide in fermented products, flavoring and related foods by gas chromatography–triple quadrupole mass spectrometry. Food Control 43:251–257
Omar MMA, Ibrahim WAW, Elbashir AA (2014) Sol–gel hybrid methyltrimethoxysilane-tetraethoxysilane as a new dispersive solid-phase extraction material for acrylamide determination in food with direct gas chromatography–mass spectrometry analysis. Food Chem 158:302–309
Omar MMA, Elbashir AA, Schmitz OJ (2015) Determination of acrylamide in Sudanese food by high performance liquid chromatography coupled with LTQ Orbitrap mass spectrometry. Food Chem 176:342–349
Russo MV et al (2014) Rapid and simple determination of acrylamide in conventional cereal-based foods and potato chips through conversion to 3-[bis(trifluoroethanoyl)amino]-3-oxopropyl trifluoroacetate by gas chromatography coupled with electron capture and ion trap mass spectrometry detectors. Food Chem 146:204–211
Sun S, Fang Y, Xia Y (2012) A facile of acrylamide in starchy food by using a solid extraction-GC strategy. Food Control 26:220–222
U.S. Environmental Protection Agency (EPA) (1994) EPA 749-F-94-005a: Chemical summary for acrylamide
Wang H et al (2013) HPLC-UV quantitative analysis of acrylamide in baked and deep-fried Chinese foods. J Food Compos Anal 31:7–11
Acknowledgments
This work was supported by National Centre for Quality Supervision and Testing of Processed Food (Guangzhou) in China. At the same time, the authors would like to thank all the workers for sampling, sample preparation and measurement.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of Interest
Cong Zhang declares that he has no conflict of interest. Yanping Xian declares that she has no conflict of interest. Xindong Guo declares that he has no conflict of interest. Huidi Liu declares that he has no conflict of interest. Hao Dong declares that he has no conflict of interest. Zhiqing Xun declares that he has no conflict of interest. Jinfeng Huang declares that she has no conflict of interest. Xiang Feng declares that he has no conflict of interest.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Informed Consent
Informed consent was obtained from all individual participants included in the study.
Rights and permissions
About this article
Cite this article
Zhang, C., Xian, Y., Guo, X. et al. Isotope Internal Standard Method for Determination of Four Acrylamide Compounds in Food Contact Paper Products and Food Simulants by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry. Food Anal. Methods 9, 1895–1903 (2016). https://doi.org/10.1007/s12161-015-0373-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12161-015-0373-6