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Optimization of Extraction of Bioactive Compounds from Black Carrot Using Response Surface Methodology (RSM)

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Abstract

In this study, optimum conditions for the extraction of black carrot anthocyanins were determined by response surface methodology. Central composite design of extraction factors (pH 2.5–6.5, temperature 4–72 °C, solvent/solid ratio 5:1–25:1 v/w, ethanol/water ratio 0:100–100:0 v/v) was generated as two replicates. Total phenolic content, total monomeric anthocyanin content, polymeric color, total antioxidant activity, and anthocyanin composition determined by high-performance liquid chromatography were used as responses. Except for color analysis, higher temperature, solid/solvent ratio, and ethanol concentration were observed to increase the extraction yield. However, polymeric color results were found to have minimum values at lower pH and solid/solvent ratio, lower or moderate temperature, and higher ethanol concentration. Optimum extraction conditions were found as follows: 50 °C, pH 3.5, solvent solid ratio 10:1 (v/w), and ethanol/water ratio 75:25 (v/v) when all responses were considered. The validation of the optimum conditions for black carrot extraction was performed at specified values.

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Acknowledgements

This research was supported by the General Directorate of Agricultural Research and Policies (TAGEM/14/ARGE-31) and by the Istanbul Technical University, Scientific Research Projects (BAP) Unit (BAP Project Number: 35097).

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Authors and Affiliations

  1. Faculty of Chemical and Metallurgical Engineering, Department of Food Engineering, Istanbul Technical University, 34469 Maslak, Istanbul, Turkey

    Burcu Guldiken, Dilek Boyacioglu & Esra Capanoglu

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  1. Burcu Guldiken
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  2. Dilek Boyacioglu
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  3. Esra Capanoglu
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Correspondence to Esra Capanoglu.

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Burcu Guldiken declares that she has no conflict of interest. Dilek Boyacioglu declares that she has no conflict of interest. Esra Capanoglu declares that she has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Guldiken, B., Boyacioglu, D. & Capanoglu, E. Optimization of Extraction of Bioactive Compounds from Black Carrot Using Response Surface Methodology (RSM). Food Anal. Methods 9, 1876–1886 (2016). https://doi.org/10.1007/s12161-015-0370-9

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  • DOI: https://doi.org/10.1007/s12161-015-0370-9

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