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Determination of Bisphenol A in Food and Environmental Samples Using Combined Solid-Phase Extraction–Dispersive Liquid–Liquid Microextraction with Solidification of Floating Organic Drop Followed by HPLC

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Abstract

A solid-phase extraction (SPE) coupled with dispersive liquid–liquid microextraction based on the solidification of floating organic drop (DLLME-SFO) method has been developed for extraction and determination of organic bisphenol A (BPA) in different real samples. Bisphenol A was determined by high-performance liquid chromatography-fluorescence detector (HPLC-FL). Variables affecting the performance of both steps were thoroughly investigated. The analytical characteristics of the method were determined. The calibration graph is linear in the range of 0.005–10 ng g−1 with detection limit of 0.002 ng g−1. The relative standard deviation (RSD) for of BPA in different samples are in the range of 6.8–9.6 % (n = 5). The obtained results show that SPE-DLLME-SFO combined with HPLC-FL is a fast and simple method for the determination of BPA in food and environmental samples.

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Acknowledgments

The authors thank the research council of Razi University of Kermanshah (Iran) for the financial support.

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Conflict of Interest

Marzieh Sadeghi has received research grants from the Department of Chemistry, Razi University, Kermanshah, Iran. Ziba Nematifar declares that he has no conflict of interest. Nazir Fattahi declares that he has no conflict of interest. Meghdad Pirsaheb declares that he has no conflict of interest. Mojtaba Shamsipur declares that he has no conflict of interest.

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This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Department Chemistry, Razi University, Kermanshah, Iran

    Marzieh Sadeghi, Ziba Nematifar & Mojtaba Shamsipur

  2. Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah, Iran

    Nazir Fattahi & Meghdad Pirsaheb

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  1. Marzieh Sadeghi
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  2. Ziba Nematifar
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  3. Nazir Fattahi
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  4. Meghdad Pirsaheb
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  5. Mojtaba Shamsipur
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Correspondence to Marzieh Sadeghi.

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Sadeghi, M., Nematifar, Z., Fattahi, N. et al. Determination of Bisphenol A in Food and Environmental Samples Using Combined Solid-Phase Extraction–Dispersive Liquid–Liquid Microextraction with Solidification of Floating Organic Drop Followed by HPLC. Food Anal. Methods 9, 1814–1824 (2016). https://doi.org/10.1007/s12161-015-0357-6

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  • DOI: https://doi.org/10.1007/s12161-015-0357-6

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