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Breed-Specific Detection of Mangalica Meat in Food Products

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Abstract

A fast and reliable diagnostic system has been developed for the detection of Mangalica meat in foods. This qualitative test is based on a recombinase polymerase amplification which can be performed on the field, in situ, where it may be necessary to determine Mangalica content in food products at once. The required equipments for the procedure are pipettes, a portable homogenizer and a portable thermostat. DNA amplification is carried out at a constant temperature, and the detection is based on antibody reaction. The detection limit is one copy of the target sequence in 1 μl reaction volume. The test can be used for uncovering falsification of local brands on the spot within a very short (25–45 min) period of time. The present approach can be adopted for the detection of other food ingredients, if the species-specific target DNA sequence is known, e.g. in case of chicken, turkey, horse, and cattle.

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Acknowledgments

The research was supported by the Ministry of Rural Development of the Hungarian Government (AHT number 334740).

Conflict of Interest

R. Szántó-Egész declares no conflict of interest. A. Jánosi declares no conflict of interest. A. Mohr, G. Szalai declares no conflict of interest. E. Koppányné Szabó declares no conflict of interest. A. Micsinai declares no conflict of interest. R. Sipos declares no conflict of interest. J. Rátky declares no conflict of interest. I. Anton declares no conflict of interest. A. Zsolnai declares no conflict of interest.

Ethical Approval

All applicable international, national and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

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Authors and Affiliations

  1. Biomi Ltd., Szent-Györgyi Albert út 4., Gödöllő, 2100, Hungary

    R. Szántó-Egész, A. Mohr, A. Micsinai & R. Sipos

  2. NARIC-Food Science Research Institute, Herman Ottó út 15., Budapest, 1022, Hungary

    A. Jánosi & E. Koppányné Szabó

  3. Peromyscus Genetic Stock Center, University of South Carolina, 715 Sumter Street, Columbia, SC, 29208, USA

    G. Szalai

  4. NARIC-Research Institute for Animal Breeding Nutrition and Meat Science, Gesztenyes u. 1., Herceghalom, 2053, Hungary

    J. Rátky, I. Anton & A. Zsolnai

Authors
  1. R. Szántó-Egész
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  2. A. Jánosi
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  3. A. Mohr
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  4. G. Szalai
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  5. E. Koppányné Szabó
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  6. A. Micsinai
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  7. R. Sipos
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  8. J. Rátky
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  9. I. Anton
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  10. A. Zsolnai
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Corresponding author

Correspondence to A. Zsolnai.

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Szántó-Egész, R., Jánosi, A., Mohr, A. et al. Breed-Specific Detection of Mangalica Meat in Food Products. Food Anal. Methods 9, 889–894 (2016). https://doi.org/10.1007/s12161-015-0261-0

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  • DOI: https://doi.org/10.1007/s12161-015-0261-0

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