Abstract
In order to identify the geographical origin of pure milk, different proteins and milk water were extracted from pure milk originated from Australia and New Zealand, Germany and France, the USA, and China. Then, δ 13C and δ 15N values of the extracted proteins and δ 2H and δ 18O values of milk water were determined by element analyzer-isotope ratio mass spectrometry (EA-IRMS). The results indicated that pure milk from these regions could be potentially discriminated by using analyte δ 13C, δ 15N, δ 2H, and δ 18O. The P value analysis of them was highly significant (P < 0.01) considering the origins. 3D distribution of δ 13C, δ 15N, δ 2H, and δ 18O can also clearly present regional concentration phenomena according to the regions of pure milk. Therefore, it can be concluded that δ 13C, δ 15N, δ 2H, and δ 18O analysis could potentially be rapid and routine for origin discrimination of pure milk.
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Acknowledgments
This work was supported by science and technology project of Quality and Technical Supervision of Guangdong Province (2011ZS01) and science and technology plan project of Guangzhou City (2011J2200016). At the same time, the authors would like to thank all the workers for sampling, sample preparation, and measurement.
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Donghui Luo declares that he has no conflict of interest. Hao Dong declares that he has no conflict of interest. Haiying Luo declares that she has no conflict of interest. Yanping Xian declares that she has no conflict of interest. Xindong Guo declares that he has no conflict of interest. Yuluan Wu declares that she has no conflict of interest. This article does not contain any studies with human or animal subjects.
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Hao Dong and Haiying Luo contributed equally to this work.
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Luo, D., Dong, H., Luo, H. et al. Multi-Element (C, N, H, O) Stable Isotope Ratio Analysis for Determining the Geographical Origin of Pure Milk from Different Regions. Food Anal. Methods 9, 437–442 (2016). https://doi.org/10.1007/s12161-015-0204-9
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DOI: https://doi.org/10.1007/s12161-015-0204-9