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Phenolic and Anthocyanin Profile of Valea Calugareasca Red Wines by HPLC-PDA-MS and MALDI-TOF Analysis

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Abstract

The quality of red wines is given by phenolic compounds and anthocyanins and is associated with colour, taste and therapeutic effects on human health. This work aims to provide a detailed profile of phenolic compounds and anthocyanins that are found in five red wine samples from Dealu Mare-Valea Calugareasca region. The phenolic and anthocyanin profiles of the red wine samples were determined by high-performance liquid chromatography-photodiode array-mass spectrometry and matrix-assisted laser desorption/ionisation–time of flight, respectively. The results obtained showed that Feteasca Neagra had the highest content of phenolic compounds followed by Pinot Noir while the preponderant compound was gallic acid. Amongst anthocyanins, malvidin was found to be the major compound and the highest anthocyanin content was found also for Feteasca Neagra wine. A simple high-performance liquid chromatography-photodiode array-mass spectrometry method was developed, optimised and applied for the quantification of phenolic compounds in red wine samples from Dealu Mare-Valea Calugareasca region. In the same time, a rapid matrix-assisted laser desorption/ionisation–time of flight method that does not need sample preparation was applied for the identification of anthocyanins. Moreover, the phenolic and anthocyanin composition of red wines from Dealu Mare-Valea Calugareasca region is reported for the first time. The phenolic and anthocyanin profile determination will be beneficial for the Romanian winemakers to produce high-quality red wines.

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Acknowledgments

This work was supported by a grant of the Romanian National Authority for Scientific Research, CNDI–UEFISCDI, project number PN-II-PT-PCCA-2011-3.1-1809.

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Conflict of Interest

All authors declare that they have no conflict of interest. Andreia Alecu declares that he has no conflict of interest. Camelia Albu declares that he has no conflict of interest. Simona Carmen Litescu declares that he has no conflict of interest. Sandra Anamaria Victoria Eremia declares that he has no conflict of interest. Gabriel Lucian Radu declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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  1. National Institute of Research and Development for Biological Sciences-Bucharest, 296 Splaiul Independentei, 060031, Bucharest, Romania

    Andreia Alecu, Camelia Albu, Simona Carmen Litescu, Sandra A. V. Eremia & Gabriel Lucian Radu

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  1. Andreia Alecu
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Correspondence to Camelia Albu.

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Alecu, A., Albu, C., Litescu, S.C. et al. Phenolic and Anthocyanin Profile of Valea Calugareasca Red Wines by HPLC-PDA-MS and MALDI-TOF Analysis. Food Anal. Methods 9, 300–310 (2016). https://doi.org/10.1007/s12161-015-0197-4

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